Comparative protein profiling identifies elongation factor-1beta and tryparedoxin peroxidase as factors associated with metastasis in Leishmania guyanensis.

Détails

ID Serval
serval:BIB_8B1C88A8B619
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Comparative protein profiling identifies elongation factor-1beta and tryparedoxin peroxidase as factors associated with metastasis in Leishmania guyanensis.
Périodique
Molecular and Biochemical Parasitology
Auteur(s)
Walker J., Acestor N., Gongora R., Quadroni M., Segura I., Fasel N., Saravia N.G.
ISSN
0166-6851 (Print)
ISSN-L
0166-6851
Statut éditorial
Publié
Date de publication
2006
Peer-reviewed
Oui
Volume
145
Numéro
2
Pages
254-264
Langue
anglais
Résumé
Parasites of the Leishmania Viannia subgenus are major causative agents of mucocutaneous leishmaniasis (MCL), a disease characterised by parasite dissemination (metastasis) from the original cutaneous lesion to form debilitating secondary lesions in the nasopharyngeal mucosa. We employed a protein profiling approach to identify potential metastasis factors in laboratory clones of L. (V.) guyanensis with stable phenotypes ranging from highly metastatic (M+) through infrequently metastatic (M+/M-) to non-metastatic (M-). Comparison of the soluble proteomes of promastigotes by two-dimensional electrophoresis revealed two abundant protein spots specifically associated with M+ and M+/M- clones (Met2 and Met3) and two others exclusively expressed in M- parasites (Met1 and Met4). The association between clinical disease phenotype and differential expression of Met1-Met4 was less clear in L. Viannia strains from mucosal (M+) or cutaneous (M-) lesions of patients. Identification of Met1-Met4 by biological mass spectrometry (LC-ES-MS/MS) and bioinformatics revealed that M+ and M- clones express distinct acidic and neutral isoforms of both elongation factor-1 subunit beta (EF-1beta) and cytosolic tryparedoxin peroxidase (TXNPx). This interchange of isoforms may relate to the mechanisms by which the activities of EF-1beta and TXNPx are modulated, and/or differential post-translational modification of the gene product(s). The multiple metabolic functions of EF-1 and TXNPx support the plausibility of their participation in parasite survival and persistence and thereby, metastatic disease. Both polypeptides are active in resistance to chemical and oxidant stress, providing a basis for further elucidation of the importance of antioxidant defence in the pathogenesis underlying MCL.
Mots-clé
Amino Acid Sequence, Animals, Computational Biology, DNA, Protozoan, Electrophoresis, Gel, Two-Dimensional, Gene Expression Regulation, Immunoblotting, Leishmania guyanensis/chemistry, Leishmania guyanensis/genetics, Mass Spectrometry, Molecular Sequence Data, Peptide Elongation Factor 1/analysis, Peroxidases/analysis, Protein Isoforms/analysis, Proteome/analysis, Protozoan Proteins/analysis, Sequence Analysis, DNA, Sequence Homology, Amino Acid
Pubmed
Web of science
Création de la notice
24/01/2008 16:02
Dernière modification de la notice
03/03/2018 19:11
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