Characterization of the staphylococcal cassette chromosome mec insertion site in 108 isolates lacking the mecA gene and identified as methicillin-resistant Staphylococcus aureus by the Xpert MRSA assay.

Détails

ID Serval
serval:BIB_8AEACD4DE859
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of the staphylococcal cassette chromosome mec insertion site in 108 isolates lacking the mecA gene and identified as methicillin-resistant Staphylococcus aureus by the Xpert MRSA assay.
Périodique
European Journal of Clinical Microbiology and Infectious Diseases
Auteur(s)
Stojanov M., Blanc D.S.
ISSN
1435-4373 (Electronic)
ISSN-L
0934-9723
Statut éditorial
Publié
Date de publication
11/2014
Peer-reviewed
Oui
Volume
33
Numéro
11
Pages
1967-1971
Langue
anglais
Notes
Publication types: Journal Article
Résumé
During a 3-year period, 848 patients were detected as carriers of methicillin-resistant Staphylococcus aureus (MRSA) by the Xpert MRSA assay (Cepheid). Among them, 108 patients (12.7 %) were colonized with strains showing methicillin-susceptible phenotypes and absence of the mecA gene, despite being positive with the rapid polymerase chain reaction (PCR) assay. DNA sequences of the staphylococcal cassette chromosome mec (SCCmec) insertion site of these "false-positive" strains was determined by direct sequencing of the genomic DNA. More than half (53.7 %) of the strains had DNA sequences unrelated to either SCC or SCCmec and one-third had DNA sequences related to non-mec SCC. Only 10.2 % of the strains carried sequences related to SCCmec, suggesting that a sequence containing the mecA gene was lost from an SCCmec. These findings differ from the general idea that all methicillin-susceptible S. aureus having positive Xpert MRSA assay results are essentially MRSA that lost the mecA gene.
Pubmed
Web of science
Création de la notice
26/01/2015 12:34
Dernière modification de la notice
20/08/2019 14:49
Données d'usage