Bone marrow dosimetry in rats using direct tissue counting after injection of radio-iodinated intact monoclonal antibodies or F(ab')2 fragments.

Détails

ID Serval
serval:BIB_8AD39F9E6F70
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Bone marrow dosimetry in rats using direct tissue counting after injection of radio-iodinated intact monoclonal antibodies or F(ab')2 fragments.
Périodique
Journal of Nuclear Medicine
Auteur(s)
Buchegger F., Chalandon Y., Pèlegrin A., Hardman N., Mach J.P.
ISSN
0161-5505 (Print)
ISSN-L
0161-5505
Statut éditorial
Publié
Date de publication
1991
Peer-reviewed
Oui
Volume
32
Numéro
7
Pages
1414-1421
Langue
anglais
Résumé
Normal rats were injected intravenously with 131I- and 125I-labeled intact murine and chimeric mouse-human monoclonal antibodies directed against carcinoembryonic antigen or with the corresponding F(ab')2 fragments. At different times after injection, individual animals were killed and radioactivity of blood and major organs, including bones and bone marrow, was determined. Ratios comparing radioactivity concentration in different tissues with that of bone marrow were calculated and found to remain stable during several effective half-lives of the antibodies. Mean bone marrow radioactivity was 35% (range, 29%-40%) of that of blood and 126% (range, 108%-147%) of that of liver after injection of intact Mabs or F(ab')2 fragments. In nude rats bearing human colon carcinoma xenografts producing carcinoembryonic antigen, relative bone marrow radioactivity was slightly lower than that in normal rats.
Mots-clé
Animals, Antibodies, Monoclonal/administration & dosage, Antibodies, Monoclonal/pharmacokinetics, Bone Marrow/metabolism, Carcinoembryonic Antigen/immunology, Colonic Neoplasms/metabolism, Female, Humans, Immunoglobulin Fab Fragments/administration & dosage, Immunoglobulin Fab Fragments/pharmacokinetics, Injections, Intravenous, Iodine Radioisotopes/diagnostic use, Mice, Radiation Dosage, Rats, Rats, Nude, Tissue Distribution, Transplantation, Heterologous
Pubmed
Web of science
Création de la notice
25/01/2008 12:27
Dernière modification de la notice
03/03/2018 19:10
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