Inhibition of complement alternative pathway in mice with Fab antibody to recombinant adipsin/factor D

Détails

ID Serval
serval:BIB_8A75672CE165
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Inhibition of complement alternative pathway in mice with Fab antibody to recombinant adipsin/factor D
Périodique
European Journal of Immunology
Auteur(s)
Pascual  M., Catana  E., White  T., Spiegelman  B. M., Schifferli  J. A.
ISSN
0014-2980
Statut éditorial
Publié
Date de publication
06/1993
Peer-reviewed
Oui
Volume
23
Numéro
6
Pages
1389-92
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jun
Résumé
Mouse adipsin is a serine protease secreted mainly by adipocytes. Similarly to factor D of human complement, it cleaves factor B. That adipsin is the equivalent of human factor D in the mouse is further suggested by their structural homology. Specific antisera against recombinant mouse adipsin (r-adipsin) were produced in rabbits. Anti-r-adipsin IgG was shown to bind to radiolabeled r-adipsin and to inhibit its hemolytic activity. In vitro, these antibodies Ab and Fab fragments thereof inhibited the adipsin/factor D hemolytic activity of mouse serum. They also blocked C3 activation induced by cobra venom factor (CVF), but did not interfere with classical pathway function. After intravenous injection of anti-r-adipsin Fab into BALB/c mice, the adipsin/factor D hemolytic activity of serum was abolished during a 4-h period. The C3 depleting effect of CVF injected intravenously was significantly delayed in BALB/c mice which had been pretreated with anti-r-adipsin Fab. These experiments demonstrate that mouse adipsin is the only form of mouse factor D and that anti-r-adipsin antibody can be used to produce a specific inhibition of the alternative pathway in vivo.
Mots-clé
Animals Complement C3/metabolism Complement Factor D/antagonists & inhibitors/*metabolism *Complement Pathway, Alternative Humans Immunoglobulin Fab Fragments/immunology Mice Mice, Inbred BALB C Recombinant Proteins Serine Endopeptidases/*immunology
Pubmed
Web of science
Création de la notice
29/01/2008 14:53
Dernière modification de la notice
03/03/2018 19:09
Données d'usage