Hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of l-Lactate in Vivo.

Détails

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Etat: Public
Version: de l'auteur
ID Serval
serval:BIB_8A3E59B47AC1
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Hyperpolarized <sup>13</sup>C Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of l-Lactate in Vivo.
Périodique
ACS chemical neuroscience
Auteur(s)
Takado Y., Cheng T., Bastiaansen JAM, Yoshihara HAI, Lanz B., Mishkovsky M., Lengacher S., Comment A.
ISSN
1948-7193 (Electronic)
ISSN-L
1948-7193
Statut éditorial
Publié
Date de publication
21/11/2018
Peer-reviewed
Oui
Volume
9
Numéro
11
Pages
2554-2562
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
The dynamics of l-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized <sup>13</sup> C magnetic resonance spectroscopy (MRS). Following the intravenous injection of hyperpolarized [1- <sup>13</sup> C]lactate, we observed that the distribution of the <sup>13</sup> C label between lactate and pyruvate, which has been shown to be representative of their pool size ratio, is different in NMRI and C57BL/6 mice, the latter exhibiting a higher level of cerebral lactate dehydrogenase A ( Ldha) expression. On the basis of this observation, and an additional set of experiments showing that the cerebral conversion of [1- <sup>13</sup> C]lactate to [1- <sup>13</sup> C]pyruvate increases after exposing the brain to ultrasound irradiation that reversibly opens the BBB, we concluded that lactate transport is rate-limited by the BBB, with a 30% increase in lactate uptake after its disruption. It was also deduced from these results that hyperpolarized <sup>13</sup> C MRS can be used to detect a variation in cerebral lactate uptake of <40 nmol in a healthy brain during an in vivo experiment lasting only 75 s, opening new opportunities to study the role of lactate in brain metabolism.
Mots-clé
Cell Biology, Biochemistry, Physiology, Cognitive Neuroscience, General Medicine, Hyperpolarization, bicarbonate, dynamic nuclear polarization, magnetic resonance spectroscopy, pyruvate, ultrasound
Pubmed
Web of science
Création de la notice
03/07/2018 11:56
Dernière modification de la notice
20/08/2019 15:49
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