Staphylococcus aureus clfB and spa alleles of the repeat regions are segregated into major phylogenetic lineages.

Détails

ID Serval
serval:BIB_8A35A6797D66
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Staphylococcus aureus clfB and spa alleles of the repeat regions are segregated into major phylogenetic lineages.
Périodique
Infection, genetics and evolution
Auteur(s)
Basset P., Hammer N.B., Kuhn G., Vogel V., Sakwinska O., Blanc D.S.
ISSN
1567-7257[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
9
Numéro
5
Pages
941-947
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To reliably differentiate among Staphylococcus aureus isolates we recently developed the Double Locus Sequence Typing (DLST) based on the analysis of partial sequences of clfB and spa genes. This method is highly discriminatory and gives unambiguous definition of types. The highly clonal population structure of S. aureus suggests that isolates with identical clfB or spa alleles belong to the same clonal complex (CC) defined by Multi-Locus Sequence Typing (MLST). To test this hypothesis as well as to investigate putative intra-CC genetic structure, we analyzed a total of 289 isolates (186 MSSA and 103 MRSA) with DLST-, spa- and MLST-typing. Among the 289 strains, 242 were clustered into 7 major MLST CCs, 40 into minor CCs and 7 were not grouped into CCs. A total of 205 DLST- and 129 spa-types were observed. With one exception, all DLST-clfB, DLST-spa and spa-type alleles were segregated into CCs. DLST-types sharing an identical allele (clfB or spa) were clustered using eBURST. Except for one strain, all isolates from each DLST cluster belonged to the same CC. However, using both DLST- and spa-typing we were not able to disclose a clear intra-CC structure. Nevertheless, the high diversity of these loci confirmed that they are good markers for local epidemiological investigations.
Mots-clé
Adhesins, Bacterial/genetics, Alleles, Antigens, Bacterial/genetics, Cluster Analysis, DNA Fingerprinting, Genes, Bacterial/genetics, Humans, Methicillin-Resistant Staphylococcus aureus/classification, Methicillin-Resistant Staphylococcus aureus/genetics, Phylogeny, Repetitive Sequences, Nucleic Acid/genetics, Sequence Analysis, DNA, Staphylococcal Infections/microbiology, Staphylococcus aureus/classification, Staphylococcus aureus/genetics
Pubmed
Web of science
Création de la notice
08/10/2009 15:10
Dernière modification de la notice
03/03/2018 19:09
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