Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure.

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_89DABB9357FA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure.
Périodique
Journal of Immunology
Auteur(s)
Miconnet Isabelle, Marrau Angélique, Farina Alex, Taffé Patrick, Vigano Selena, Harari Alexandre, Pantaleo Giuseppe
ISSN
1550-6606 (Electronic)
ISSN-L
0022-1767
Statut éditorial
Publié
Date de publication
2011
Peer-reviewed
Oui
Volume
186
Numéro
12
Pages
7039-7049
Langue
anglais
Résumé
Ex vivo analysis of virus-specific CD8 T cell populations by anchored PCR has shown that the CD8 TCR repertoire was less oligoclonal (seven to nine clonotypes per individual epitope) than previously thought. In the current study, TCR diversity was investigated by assessing both the overall TCR β-chain variable regions usage as well as the CDR3 regions in ex vivo-isolated CMV- and EBV-specific CD8 T cells from 27 healthy donors. The average number of clonotypes specific to most single viral epitopes comprised between 14 and 77. Changes in the CD8 TCR repertoire were also longitudinally assessed under conditions of HIV-1 chronic infection (i.e., in patients with suppressed virus replication and after treatment interruption and Ag re-exposure). The results showed that a large renewal (≤80%) of the TRB repertoire occurred after Ag re-exposure and was eventually associated with an increased T cell recognition functional avidity. These results demonstrate that the global CD8 TCR repertoire is much more diverse (≤9-fold) than previously estimated and provide the mechanistic basis for supporting massive repertoire renewal during chronic virus infection and Ag re-exposure.
Pubmed
Web of science
Création de la notice
08/06/2011 11:18
Dernière modification de la notice
03/03/2018 19:08
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