[Ca2+] modulates the ratio between cycloxygenase and lipoxygenase metabolism of arachidonic acid in homogenates of hippocampal astroglial cultures.

Détails

ID Serval
serval:BIB_89C333D4D2BF
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
[Ca2+] modulates the ratio between cycloxygenase and lipoxygenase metabolism of arachidonic acid in homogenates of hippocampal astroglial cultures.
Périodique
Neuroscience Letters
Auteur(s)
Volterra A., Trotti D., Bezzi P., Civenni G., Racagni G.
ISSN
0304-3940 (Print)
ISSN-L
0304-3940
Statut éditorial
Publié
Date de publication
1995
Volume
183
Numéro
3
Pages
160-163
Langue
anglais
Résumé
While studying the enzymatic processing of arachidonic acid (AA) to eicosanoids in homogenates of hippocampal astrocytes, we observed that all the HPLC peaks corresponding to AA metabolites displayed significantly different levels depending on the presence or not of free Ca2+ in the incubation medium. A specific pattern was noticed, i.e. lipoxygenase (LOX) derivatives, in particular 12-hydroxyeicosatetraenoic acid (12-HETE), showed higher levels in medium containing 1 mM Ca2+, while cycloxygenase (COX) products including prostaglandins (PG) F2 alpha, E2 and D2 and 12-hydroxyhepatadecatrienoic acid (12-HHT), were higher in Ca(2+)-free medium. COX metabolism exceeded LOX metabolism by threefold in Ca(2+)-free medium, while it was only 60% of it in 1 mM Ca2+. The total amount of AA processed under the two conditions was identical. These data suggest that free [Ca2+] influences the pattern of AA metabolites formed in hippocampal astrocytes, with possible important implications in view of the distinct roles played by COX and LOX eicosanoids in synaptic transmission and neurotoxicity in this area.
Mots-clé
Animals, Arachidonic Acids/metabolism, Astrocytes/enzymology, Astrocytes/metabolism, Calcium/physiology, Cells, Cultured, Culture Media, Eicosanoids/metabolism, Hippocampus/cytology, Hippocampus/enzymology, Lipoxygenase/metabolism, Prostaglandin-Endoperoxide Synthases/metabolism, Rats
Pubmed
Web of science
Création de la notice
24/01/2008 15:37
Dernière modification de la notice
03/03/2018 19:08
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