Simultaneous identification of multiple mammalian species from mixed forensic samples based on mtDNA control region length polymorphism

Détails

ID Serval
serval:BIB_89743BE7C3BA
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Etude de cas (case report): rapporte une observation et la commente brièvement.
Collection
Publications
Titre
Simultaneous identification of multiple mammalian species from mixed forensic samples based on mtDNA control region length polymorphism
Périodique
Forensic Science International : Genetics Supplement Series
Auteur(s)
Fumagalli L., Jan Cabrita C., Castella V.
ISSN
1875-1768
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
2
Numéro
1
Pages
302-303
Langue
anglais
Résumé
Molecular species identification in mixed or contaminated biological material has always been problematic. We developed a simple and accurate method for mammal DNA identification in mixtures, based on interspecific mitochondrial DNA control region length polymorphism. Contrary to other published methods dealing with species mixtures, our protocol requires a single universal primer pair and amplification step, and is not based on a pre-defined panel of species. This protocol has been routinely employed by our laboratory for species identification in dozens of human and animal forensic caseworks. Six representative forensic caseworks involving the specific identification of mixed animal samples are reported in this paper, in order to demonstrate the applicability and usefulness of the method.
Mots-clé
DNA mixtures, Species identification, Wildlife forensics, Mitochondrial DNA control region
Création de la notice
17/08/2009 16:19
Dernière modification de la notice
20/08/2019 15:48
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