Association between intraepithelial Escherichia coli and colorectal cancer

Details

Serval ID
serval:BIB_88FFA3295749
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Association between intraepithelial Escherichia coli and colorectal cancer
Journal
Gastroenterology
Author(s)
Swidsinski  A., Khilkin  M., Kerjaschki  D., Schreiber  S., Ortner  M., Weber  J., Lochs  H.
ISSN
0016-5085 (Print)
Publication state
Published
Issued date
08/1998
Volume
115
Number
2
Pages
281-6
Notes
Journal Article --- Old month value: Aug
Abstract
BACKGROUND & AIMS: Although multiple studies have focused on Helicobacter pylori, little is known about the mucosa-associated flora of the colon. The aim of this study was to detect bacteria directly in colonic mucosa from patients screened for colorectal cancer. METHODS: Bacteria were quantified with the polymerase chain reaction and identified by comparative sequence analysis in colonoscopic biopsy specimens from 31 asymptomatic and 34 symptomatic controls with normal colonoscopic findings, 29 patients with colonic adenoma, and 31 patients with colorectal carcinoma. In 41 patients, intra- and extracellular location of bacteria was confirmed with the gentamicin protection assay. RESULTS: No bacteria were detected in biopsy specimens from 97% of asymptomatic and 69% of symptomatic controls. In contrast, bacterial concentrations of 10(3)-10(5) colony-forming units per microliter were detected in biopsy specimens from both malignant and macroscopically normal tissue in 90% and 93% of patients with adenoma and carcinoma, respectively. E. coli and coli-like bacteria were shown to colonize the colonic mucosa in 82% of these patients. The gentamicin protection assay indicated that E. coli was partially intracellular in 87% of patients with adenoma and carcinoma and in none of the controls. CONCLUSIONS: The colonic mucosa of patients with colorectal carcinoma but not normal colonic mucosa is colonized by intracellular E. coli.
Keywords
Adenoma/*microbiology/pathology Adult Aged Aged, 80 and over Biopsy Carcinoma/*microbiology/pathology Colon/*microbiology/pathology Colonoscopy Colorectal Neoplasms/*microbiology/pathology Escherichia coli/*isolation & purification Female Fluorescent Antibody Technique, Indirect Humans Intestinal Mucosa/*microbiology/pathology Male Middle Aged Reference Values
Pubmed
Web of science
Create date
25/01/2008 17:08
Last modification date
20/08/2019 15:48
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