In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.

Détails

Ressource 1Télécharger: BIB_88212B28C49F.P001.pdf (1013.83 [Ko])
Etat: Serval
Version: Author's accepted manuscript
ID Serval
serval:BIB_88212B28C49F
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vitro and in vivo ocular biocompatibility of electrospun poly(ɛ-caprolactone) nanofibers.
Périodique
European Journal of Pharmaceutical Sciences
Auteur(s)
Da Silva G.R., Lima T.H., Oréfice R.L., Fernandes-Cunha G.M., Silva-Cunha A., Zhao M., Behar-Cohen F.
ISSN
1879-0720 (Electronic)
ISSN-L
0928-0987
Statut éditorial
Publié
Date de publication
2015
Peer-reviewed
Oui
Volume
73
Pages
9-19
Langue
anglais
Résumé
Biocompatibility is a requirement for the development of nanofibers for ophthalmic applications. In this study, nanofibers were elaborated using poly(ε-caprolactone) via electrospinning. The ocular biocompatibility of this material was investigated. MIO-M1 and ARPE-19 cell cultures were incubated with nanofibers and cellular responses were monitored by viability and morphology. The in vitro biocompatibility revealed that the nanofibers were not cytotoxic to the ocular cells. These cells exposed to the nanofibers proliferated and formed an organized monolayer. ARPE-19 and MIO-M1 cells were capable of expressing GFAP, respectively, demonstrating their functionality. Nanofibers were inserted into the vitreous cavity of the rat's eye for 10days and the in vivo biocompatibility was investigated using Optical Coherence Tomography (OCT), histology and measuring the expression of pro-inflammatory genes (IL-1β, TNF-α, VEGF and iNOS) (real-time PCR). The OCT and the histological analyzes exhibited the preserved architecture of the tissues of the eye. The biomaterial did not elicit an inflammatory reaction and pro-inflammatory cytokines were not expressed by the retinal cells, and the other posterior tissues of the eye. Results from the biocompatibility studies indicated that the nanofibers exhibited a high degree of cellular biocompatibility and short-term intraocular tolerance, indicating that they might be applied as drug carrier for ophthalmic use.
Pubmed
Web of science
Open Access
Oui
Création de la notice
31/03/2015 16:28
Dernière modification de la notice
08/05/2019 21:31
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