Article: article from journal or magazin.
Developmental change in isoproterenol-mediated relaxation of pulmonary veins of fetal and newborn lambs.
Journal of Applied Physiology
beta-Adrenergic agonists are important regulators of perinatal pulmonary circulation. They cause vasodilation primarily via the adenyl cyclase-adenosine 3',5'-cyclic monophosphate (cAMP) pathway. We examined the responses of isolated fourth-generation pulmonary veins of term fetal (145 +/- 2 days gestation) and newborn (10 +/- 1 days) lambs to isoproterenol, a beta-adrenergic agonist. In vessels preconstricted with U-46619 (a thromboxane A2 analog), isoproterenol induced greater relaxation in pulmonary veins of newborn lambs than in those of fetal lambs. The relaxation was eliminated by propranolol, a beta-adrenergic antagonist. Forskolin, an activator of adenyl cyclase, also caused greater relaxation of veins of newborn than those of fetal lambs. 8-Bromoadenosine 3',5'-cyclic monophosphate, a cell membrane-permeable analog of cAMP, induced a similar relaxation of all vessels. Biochemical studies show that isoproterenol and forskolin induced a greater increase in cAMP content and in adenyl cyclase activity of pulmonary veins in the newborn than in the fetal lamb. These results demonstrate that beta-adrenergic-agonist-mediated relaxation of pulmonary veins increases with maturation. An increase in the activity of adenyl cyclase may contribute to the change.
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid/pharmacology, 8-Bromo Cyclic Adenosine Monophosphate/pharmacology, Adenylate Cyclase/analysis, Animals, Animals, Newborn/physiology, Cyclic AMP/analysis, Fetus/physiology, Forskolin/pharmacology, Isoproterenol/pharmacology, Muscle Relaxation/drug effects, Muscle, Smooth, Vascular/drug effects, Propranolol/pharmacology, Pulmonary Veins/drug effects, Sheep/growth &, development
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