Direct detection of Listeria monocytogenes using paramagnetic bead DNA extraction and enzymatic DNA amplification.

Détails

ID Serval
serval:BIB_87D431859A89
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Direct detection of Listeria monocytogenes using paramagnetic bead DNA extraction and enzymatic DNA amplification.
Périodique
Molecular and Cellular Probes
Auteur(s)
Niederhauser C., Lüthy J., Candrian U.
ISSN
0890-8508 (Print)
ISSN-L
0890-8508
Statut éditorial
Publié
Date de publication
1994
Peer-reviewed
Oui
Volume
8
Numéro
3
Pages
223-228
Langue
anglais
Notes
Publication types: Journal Article Publication Status: ppublish
Résumé
The potential of the polymerase chain reaction (PCR) as a tool for direct detection of Listeria monocytogenes in food and clinical samples was investigated. A specific oligonucleotide, directed against the listeriolysin 0 gene of L. monocytogenes, was coupled to paramagnetic beads and used to isolate listerial DNA from food homogenates and blood. The isolated DNA was amplified with a seminested PCR procedure, which recognized the hly gene. The detection limit for L. monocytogenes in 50 ml of a buffer solution was between 1 and 10 colony forming units (cfu). In food homogenates consisting of 10 g food and 40 ml 0.85% NaCl solution, artificially spiked with an overnight culture of L. monocytogenes, the detection limit was 1-10 cfu. The method was further evaluated by application to naturally contaminated food samples. In 250 microliters whole human blood artificially spiked with L. monocytogenes 10,000 cfu could be detected.
Mots-clé
Base Sequence, DNA, Bacterial/analysis, DNA, Bacterial/genetics, Food Microbiology, Gene Amplification, Humans, Immunomagnetic Separation, Listeria monocytogenes/genetics, Listeria monocytogenes/isolation & purification, Methods, Molecular Sequence Data, Polymerase Chain Reaction, Sensitivity and Specificity
Pubmed
Web of science
Création de la notice
09/11/2014 16:45
Dernière modification de la notice
03/03/2018 19:03
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