Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.

Détails

ID Serval
serval:BIB_87B7A5F8B553
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Nawrath C., Poirier Y., Somerville C.
ISSN
0027-8424 (Print)
ISSN-L
0027-8424
Statut éditorial
Publié
Date de publication
1994
Volume
91
Numéro
26
Pages
12760-12764
Langue
anglais
Résumé
In the bacterium Alcaligenes eutrophus, three genes encode the enzymes necessary to catalyze the synthesis of poly[(R)-(-)-3-hydroxybutyrate] (PHB) from acetyl-CoA. In order to target these enzymes into the plastids of higher plants, the genes were modified by addition of DNA fragments encoding a pea chloroplast transit peptide, a constitutive plant promoter, and a poly(A) addition sequence. Each of the modified bacterial genes was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation, and plants containing all three genes were obtained by sexual crosses. These plants accumulated PHB up to 14% of the dry weight as 0.2- to 0.7-micron granules within plastids. In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression of the PHB biosynthetic pathway in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB that accumulated. We conclude that there does not appear to be any biological barrier to high-level production of PHB in higher plants. The high level of PHB accumulation also suggests that the synthesis of plastid acetyl-CoA is regulated by a mechanism which responds to metabolic demand.
Mots-clé
Acyltransferases/metabolism, Aldehyde Oxidoreductases/metabolism, Amino Acid Sequence, Arabidopsis, Cell Compartmentation, Molecular Sequence Data, Plants, Genetically Modified, Plastids/metabolism, Polyesters/metabolism, Recombinant Proteins/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 20:49
Dernière modification de la notice
08/05/2019 21:30
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