Identification of proteins from Plasmodium falciparum that are homologous to reticulocyte binding proteins in Plasmodium vivax.

Détails

ID Serval
serval:BIB_8782FE7AEBB9
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Identification of proteins from Plasmodium falciparum that are homologous to reticulocyte binding proteins in Plasmodium vivax.
Périodique
Infection and Immunity
Auteur(s)
Triglia T., Thompson J., Caruana S.R., Delorenzi M., Speed T., Cowman A.F.
ISSN
0019-9567 (Print)
ISSN-L
0019-9567
Statut éditorial
Publié
Date de publication
2001
Volume
69
Numéro
2
Pages
1084-1092
Langue
anglais
Résumé
Plasmodium falciparum infections can be fatal, while P. vivax infections usually are not. A possible factor involved in the greater virulence of P. falciparum is that this parasite grows in red blood cells (RBCs) of all maturities whereas P. vivax is restricted to growth in reticulocytes, which represent only approximately 1% of total RBCs in the periphery. Two proteins, expressed at the apical end of the invasive merozoite stage from P. vivax, have been implicated in the targeting of reticulocytes for invasion by this parasite. A search of the P. falciparum genome databases has identified genes that are homologous to the P. vivax rbp-1 and -2 genes. Two of these genes are virtually identical over a large region of the 5' end but are highly divergent at the 3' end. They encode high-molecular-mass proteins of >300 kDa that are expressed in late schizonts and localized to the apical end of the merozoite. To test a potential role in merozoite invasion of RBCs, we analyzed the ability of these proteins to bind to mature RBCs and reticulocytes. No binding to mature RBCs or cell preparations enriched for reticulocytes was detected. We identified a parasite clone that lacks the gene for one of these proteins, showing that the gene is not required for normal in vitro growth. Antibodies to these proteins can inhibit merozoite invasion of RBCs.
Mots-clé
Amino Acid Sequence, Animals, Erythrocytes/metabolism, Erythrocytes/parasitology, Hemoglobins/analysis, Humans, Mice, Molecular Sequence Data, Plasmodium falciparum/chemistry, Plasmodium falciparum/genetics, Plasmodium vivax/chemistry, Protozoan Proteins/analysis, Protozoan Proteins/chemistry, Rabbits, Reticulocytes/parasitology
Pubmed
Web of science
Création de la notice
22/04/2013 9:28
Dernière modification de la notice
03/03/2018 19:02
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