Determination of plasma levels of citalopram and its demethylated and deaminated metabolites by gas chromatography and gas chromatography-mass spectrometry.

Détails

ID Serval
serval:BIB_877B784FDB7D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Determination of plasma levels of citalopram and its demethylated and deaminated metabolites by gas chromatography and gas chromatography-mass spectrometry.
Périodique
Journal of Chromatography
Auteur(s)
Reymond P., Amey M., Souche A., Lambert S., Konrat H., Eap C.B., Baumann P.
ISSN
0021-9673 (Print)
ISSN-L
0021-9673
Statut éditorial
Publié
Date de publication
1993
Peer-reviewed
Oui
Volume
616
Numéro
2
Pages
221-228
Langue
anglais
Résumé
Sensitive and specific methods based on gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) for the determination of levels of citalopram, desmethylcitalopram and didesmethylcitalopram in the plasma of patients treated with citalopram are presented, as well as a GC-MS procedure for the assay of the citalopram propionic acid derivative. After addition of a separate internal standard for each drug, liquid-solvent extraction is used to separate the basic compounds from the acid compounds. The demethylated amines are derivatized with trifluoroacetic anhydride, and the acid metabolite with methyl iodide. GC-MS is performed in the electron impact mode, as mass spectrometry by the (positive-ion) chemical ionization mode (methane and ammonia) appeared to be unsuitable. The limits of quantification were 1 ng/ml for citalopram and desmethylcitalopram and 2 ng/ml for the other metabolites. The correlation coefficients for the calibration curves (range 10-500 ng/ml) were > or = 0.999 for all compounds, whether determined by GC or GC-MS.
Mots-clé
Antidepressive Agents, Tricyclic/analysis, Chromatography, Gas, Citalopram/blood, Dealkylation, Deamination, Gas Chromatography-Mass Spectrometry, Humans, Indicators and Reagents, Reference Standards
Pubmed
Web of science
Création de la notice
30/01/2008 10:44
Dernière modification de la notice
03/03/2018 19:02
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