A global HILIC-MS approach to measure polar human cerebrospinal fluid metabolome: Exploring gender-associated variation in a cohort of elderly cognitively healthy subjects.

Détails

ID Serval
serval:BIB_8774180E947C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
A global HILIC-MS approach to measure polar human cerebrospinal fluid metabolome: Exploring gender-associated variation in a cohort of elderly cognitively healthy subjects.
Périodique
Analytica chimica acta
Auteur(s)
Gallart-Ayala H., Konz I., Mehl F., Teav T., Oikonomidi A., Peyratout G., van der Velpen V., Popp J., Ivanisevic J.
ISSN
1873-4324 (Electronic)
ISSN-L
0003-2670
Statut éditorial
Publié
Date de publication
11/12/2018
Peer-reviewed
Oui
Volume
1037
Pages
327-337
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Cerebrospinal fluid (CSF) is a key body fluid that maintains the homeostasis in central nervous system (CNS). As a biofluid whose content reflects the brain metabolic activity, the CSF has been profiled in the context of neurological diseases to provide novel insights into the disease mechanisms. However, a global high-throughput approach to measure a broad diversity of polar metabolites present in CSF is lacking. Although still perceived as challenging and less reproducible, hydrophilic interaction liquid chromatography (HILIC) has recently evolved to offer the unprecedented coverage capacity of water-soluble metabolome. Here, we present a global HILIC high-resolution mass spectrometry-based (HRMS) approach that combines the profiling in acidic pH ESI (+) and basic pH ESI (-) mode to extend the coverage of CSF polar metabolome. This approach allowed us to annotate and measure a broad range of central carbon metabolites (implicated in glycolysis, TCA cycle, nucleotide, amino acid and fatty acid metabolism) in CSF collected from cognitively healthy elderly volunteers (n = 32), using a single extraction method. Metabolite annotation was achieved using the accurate mass, RT and MS/MS criteria, allowing for the characterization of 146 measurable metabolites. Exploration of characterized individual CSF profiles allowed for a discovery of intriguing gender-associated differences, with significantly higher acylcarnitine levels in men and higher taurine levels women. With this case study, we demonstrate the value of combined HILIC ESI ± HRMS profiling to assess CSF metabolome in clinical research studies.
Mots-clé
Aged, Cerebrospinal Fluid/metabolism, Chromatography, Liquid, Cognitive Dysfunction/metabolism, Cohort Studies, Female, Healthy Volunteers, Humans, Male, Mass Spectrometry, Metabolome, Middle Aged, Sex Characteristics, CSF, Cohort study, Gender differences, HILIC, Metabolite identification, Metabolomics
Pubmed
Web of science
Création de la notice
18/06/2018 14:27
Dernière modification de la notice
21/01/2019 7:26
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