Requirement of glucose metabolism for regulation of glucose transporter type 2 (GLUT2) gene expression in liver.

Détails

ID Serval
serval:BIB_876599980A2C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Requirement of glucose metabolism for regulation of glucose transporter type 2 (GLUT2) gene expression in liver.
Périodique
Biochemical Journal
Auteur(s)
Rencurel F., Waeber G., Antoine B., Rocchiccioli F., Maulard P., Girard J., Leturque A.
ISSN
0264-6021
Statut éditorial
Publié
Date de publication
1996
Peer-reviewed
Oui
Volume
314
Numéro
3
Pages
903-909
Langue
anglais
Résumé
Previous studies have shown that glucose increases the glucose transporter (GLUT2) mRNA expression in the liver in vivo and in vitro. Here we report an analysis of the effects of glucose metabolism on GLUT2 gene expression. GLUT2 mRNA accumulation by glucose was not due to stabilization of its transcript but rather was a direct effect on gene transcription. A proximal fragment of the 5' regulatory region of the mouse GLUT2 gene linked to a reporter gene was transiently transfected into liver GLUT2-expressing cells. Glucose stimulated reporter gene expression in these cells, suggesting that glucose-responsive elements were included within the proximal region of the promoter. A dose-dependent effect of glucose on GLUT2 expression was observed over 10 mM glucose irrespective of the hexokinase isozyme (glucokinase K(m) 16 mM; hexokinase I K(m) 0.01 mM) present in the cell type used. This suggests that the correlation between extracellular glucose and GLUT2 mRNA concentrations is simply a reflection of an activation of glucose metabolism. The mediators and the mechanism responsible for this response remain to be determined. In conclusion, glucose metabolism is required for the proper induction of the GLUT2 gene in the liver and this effect is transcriptionally regulated.
Mots-clé
Acetylglucosamine, Amanitins, Animals, Cells, Cultured, Dactinomycin, Female, Gene Expression Regulation, Glucose, Glucose Transporter Type 2, Kinetics, Liver, Mice, Monosaccharide Transport Proteins, Protein Synthesis Inhibitors, Puromycin, RNA, Messenger, Rats, Rats, Wistar, Recombinant Proteins, Regulatory Sequences, Nucleic Acid, Transcription, Genetic, Transfection
Pubmed
Web of science
Création de la notice
25/01/2008 15:10
Dernière modification de la notice
03/03/2018 19:01
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