Human and simian immunodeficiency viruses deregulate early hematopoiesis through a Nef/PPARgamma/STAT5 signaling pathway in macaques.

Détails

ID Serval
serval:BIB_8754B6DFBB02
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Human and simian immunodeficiency viruses deregulate early hematopoiesis through a Nef/PPARgamma/STAT5 signaling pathway in macaques.
Périodique
Journal of Clinical Investigation
Auteur(s)
Prost S., Le Dantec M., Augé S., Le Grand R., Derdouch S., Auregan G., Déglon N., Relouzat F., Aubertin A.M., Maillere B., Dusanter-Fourt I., Kirszenbaum M.
ISSN
0021-9738 (Print)
ISSN-L
0021-9738
Statut éditorial
Publié
Date de publication
2008
Volume
118
Numéro
5
Pages
1765-1775
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov'tPublication Status: ppublish
Résumé
Infection of primates by HIV-1 and SIV induces multiple hematological abnormalities of central hematopoietic origin. Although these defects greatly contribute to the pathophysiology of HIV-1 infection, the molecular basis for altered BM function remains unknown. Here we show that when cynomolgus macaques were infected with SIV, the multipotent potential of their hematopoietic progenitor cells was lost, and this correlated with downregulation of STAT5A and STAT5B expression. However, forced expression of STAT5B entirely rescued the multipotent potential of the hematopoietic progenitor cells. In addition, an accessory viral protein required for efficient SIV and HIV replication and pathogenicity, "Negative factor" (Nef), was essential for SIV-mediated impairment of the multipotent potential of hematopoietic progenitors ex vivo and in vivo. This newly uncovered property of Nef was both conserved between HIV-1 and SIV strains and entirely dependent upon the presence of PPARgamma in targeted cells. Further, PPARgamma agonists mimicked Nef activity by inhibiting STAT5A and STAT5B expression and hampering the functionality of hematopoietic progenitors both ex vivo and in vivo. These findings have extended the role of Nef in the pathogenicity of HIV-1 and SIV and reveal a pivotal role for the PPARgamma/STAT5 pathway in the regulation of early hematopoiesis. This study may provide a basis for investigating the potential therapeutic benefits of PPARgamma antagonists in both patients with AIDS and individuals with hematopoietic disorders.
Mots-clé
Amino Acid Sequence, Animals, Female, Gene Products, nef/genetics, Gene Products, nef/metabolism, HIV-1/genetics, HIV-1/metabolism, Hematologic Diseases/metabolism, Hematologic Diseases/physiopathology, Hematopoiesis/physiology, Hematopoietic Stem Cells/cytology, Hematopoietic Stem Cells/physiology, Humans, K562 Cells, Macaca fascicularis, Male, Molecular Sequence Data, PPAR gamma/genetics, PPAR gamma/metabolism, STAT5 Transcription Factor/genetics, STAT5 Transcription Factor/metabolism, Signal Transduction/physiology, Simian immunodeficiency virus/genetics, Simian immunodeficiency virus/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
13/12/2011 17:17
Dernière modification de la notice
08/05/2019 21:29
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