Evaluating the use of whole-genome sequencing for outbreak investigations in the lack of closely related reference genome.

Détails

ID Serval
serval:BIB_871E9A950339
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Evaluating the use of whole-genome sequencing for outbreak investigations in the lack of closely related reference genome.
Périodique
Infection, genetics and evolution
Auteur(s)
Abdelbary MMH, Senn L., Moulin E., Prod'hom G., Croxatto A., Greub G., Blanc D.S.
ISSN
1567-7257 (Electronic)
ISSN-L
1567-1348
Statut éditorial
Publié
Date de publication
04/2018
Peer-reviewed
Oui
Volume
59
Pages
1-6
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Whole-genome sequencing (WGS) has emerged as a powerful molecular typing method for outbreak analysis enabling the rapid discrimination between outbreak and non-outbreak isolates. However, such analysis can be challenging in the absence of closely related reference genomes. In this study, we assessed the use of WGS in investigating an outbreak of a relatively understudied bacterial pathogen with no publicly available closely related reference genome. Eleven Burkholderia cepacia complex (Bcc) isolates (seven from patients and four from disposable dermal gloves packages) that were collected during an outbreak were sequenced using the Illumina MiSeq platform. Our results showed that mapping the 11 sequenced Bcc outbreak isolates against a genetically distant reference genome yield loses coverage (31.6-48.3%) and a high number of detected false single-nucleotide polymorphisms (SNPs) (1123-2139). Therefore, a reference genome consensus from an outbreak clinical isolate was generated by combining both de novo assembly and mapping approaches. Based on this approach, we were able to demonstrate that the Bcc outbreak isolates were closely related and were phylogenetically distinct from the 11 publically available Bcc genomes. In addition, the pairwise SNP distance analysis detected only 1 to 6 SNPs differences among the outbreak isolates, confirming that contaminated disposable dermal gloves were the cause of the outbreak.
Mots-clé
Burkholderia Infections/epidemiology, Burkholderia Infections/microbiology, Burkholderia cepacia/classification, Burkholderia cepacia/genetics, DNA, Bacterial/genetics, Disease Outbreaks, Genome, Bacterial/genetics, Humans, Molecular Epidemiology/methods, Reference Standards, Whole Genome Sequencing/methods, Whole Genome Sequencing/standards, Burkholderia cepacia complex, Outbreak investigation, Whole-genome sequencing
Pubmed
Web of science
Création de la notice
01/02/2018 22:02
Dernière modification de la notice
15/04/2019 11:00
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