In vivo fate mapping with SCL regulatory elements identifies progenitors for primitive and definitive hematopoiesis in mice.

Détails

ID Serval
serval:BIB_871CFA07DB34
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
In vivo fate mapping with SCL regulatory elements identifies progenitors for primitive and definitive hematopoiesis in mice.
Périodique
Mechanisms of Development
Auteur(s)
Bockamp E., Antunes C., Liebner S., Schmitt S., Cabezas-Wallscheid N., Heck R., Ohnngemach S., Oesch-Bartlomowicz B., Rickert C., Sanchez M.J., Hengstler J., Kaina B., Wilson A., Trumpp A., Eshkind L.
ISSN
1872-6356[electronic]
Statut éditorial
Publié
Date de publication
2009
Volume
126
Numéro
10
Pages
863-872
Langue
anglais
Résumé
One of the principal issues facing biomedical research is to elucidate developmental pathways and to establish the fate of stem and progenitor cells in vivo. Hematopoiesis, the process of blood cell formation, provides a powerful experimental system for investigating this process. Here, we employ transcriptional regulatory elements from the stem cell leukemia (SCL) gene to selectively label primitive and definitive hematopoiesis. We report that SCL-labelled cells arising in the mid to late streak embryo give rise to primitive red blood cells but fail to contribute to the vascular system of the developing embryo. Restricting SCL-marking to different stages of foetal development, we identify a second population of multilineage progenitors, proficient in contributing to adult erythroid, myeloid and lymphoid cells. The distinct lineage-restricted potential of SCL-labelled early progenitors demonstrates that primitive erythroid cell fate specification is initiated during mid gastrulation. Our data also suggest that the transition from a hemangioblastic precursors with endothelial and blood forming potential to a committed hematopoietic progenitor must have occurred prior to SCL-marking of definitive multilineage blood precursors.
Mots-clé
Stem cell leukemia gene, Conditional mouse model, Tetracycline system, CRE system, Mesodermal blood cell specification, In vivo linage and fate tracing, Primitive hematopoiesis, Definitive hematopoiesis, Blood cell precursors, In vivo lineage marking, Stem-Cell Enhancer, Mouse Embryo, Yolk-Sac, Adult Hematopoiesis, Blood Formation, Gene, Expression, Vasculogenesis, Erythropoiesis, Endothelium
Pubmed
Web of science
Open Access
Oui
Création de la notice
20/11/2009 8:35
Dernière modification de la notice
20/08/2019 14:46
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