Automated electron tomography of the septal pore cap in Rhizoctonia solani.

Détails

ID Serval
serval:BIB_870DEDE24597
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Automated electron tomography of the septal pore cap in Rhizoctonia solani.
Périodique
Journal of Structural Biology
Auteur(s)
Müller W.H., Koster A.J., Humbel B.M., Ziese U., Verkleij A.J., van Aelst A.C., van der Krift T.P., Montijn R.C., Boekhout T.
ISSN
1047-8477 (Print)
ISSN-L
1047-8477
Statut éditorial
Publié
Date de publication
2000
Volume
131
Numéro
1
Pages
10-18
Langue
anglais
Résumé
Dolipore septa and septal pore caps (SPCs) in filamentous basidiomycetes may play an important role in maintaining the integrity of hyphal cells. We have investigated the ultrastructure of the dolipore septum and the SPC in Rhizoctonia solani hyphal cells after high-pressure freezing, freeze substitution, and Spurr embedding. We visualized the SPC with associated cell ultrastructures in three dimensions by automated electron tomography of thick-sectioned cells, followed by 3D tomographic reconstructions. Using these methods we were able to document the passage of mitochondria through the SPC, small tubular membranous structures at the entrance of the septal pore channel, filamentous structures connecting the inner side of the SPC with pore-plugging material, thin filaments anchoring the pore-plugging material with the plasma membrane, small vesicles attached to the plugging material, and tubular endoplasmic reticulum continuous with the base of the SPC. We hypothesize that the SPC, the filamentous structures, the plugging material, and the endoplasmic reticulum act in a coordinated fashion to maintain cellular integrity, intercellular communication, and the transport of solutes and cell organelles in the filamentous fungus R. solani.
Mots-clé
Automation, Cell Membrane/metabolism, Cell Membrane/ultrastructure, Cryoelectron Microscopy, Electrons, Endoplasmic Reticulum/metabolism, Endoplasmic Reticulum/ultrastructure, Freezing, Image Processing, Computer-Assisted, Intercellular Junctions/metabolism, Intercellular Junctions/ultrastructure, Intracellular Membranes/metabolism, Intracellular Membranes/ultrastructure, Microscopy, Electron, Microscopy, Electron, Scanning, Mitochondria/metabolism, Mitochondria/ultrastructure, Rhizoctonia/cytology, Rhizoctonia/ultrastructure, Tomography
Pubmed
Web of science
Création de la notice
18/10/2012 15:09
Dernière modification de la notice
03/03/2018 19:00
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