Article: article from journal or magazin.
Localization of neuropeptide Y and its C-terminal flanking peptide in human renal tissue
We produced and characterized three anti-C-flanking peptides of neuropeptide Y (CPON) monoclonal antibodies. The Ka for these antibodies ranged from 0.4 to 0.8 x 10(8) l/mol with an IC50 for CPON(1-30) at about 20 nM as determined by ELISA. All these antibodies are IgG1 and recognize the 16-30 part of CPON. These antibodies and a specific anti-NPY monoclonal antibody were used to study the localization of CPON and NPY in the human kidney. The avidin-biotin technique was employed. NPY and CPON immunoreactivities were present in large amount in the renal tubules of the human kidney but not in the glomeruli. No labeling was found within the renal arterioles and veins, but some immunoreactivity was evidenced in the perivascular area. Because no specific receptor for CPON has been described to date, the presence of this peptide in the tubules may be due to a tubular reabsorption or perhaps to a local synthesis of pro-NPY.
Adrenal Gland Neoplasms/pathology Antibodies, Monoclonal Antibody Specificity Biopsy Cross Reactions Enzyme-Linked Immunosorbent Assay Graft Rejection/pathology Humans Immunoglobulin G Immunohistochemistry Kidney/*cytology/pathology Kidney Diseases/pathology Kidney Glomerulus/cytology/pathology Kidney Transplantation/pathology Kidney Tubules/cytology/pathology Neuropeptide Y/*analysis Peptide Fragments/*analysis Pheochromocytoma/pathology Sensitivity and Specificity
Web of science
Last modification date