Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.

Détails

ID Serval
serval:BIB_86FADFB4E6A7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Isolation of two closely related vitellogenin genes, including their flanking regions, from a Xenopus laevis gene library.
Périodique
Proceedings of the National Academy of Sciences of the United States of America
Auteur(s)
Wahli W., Dawid I.B.
ISSN
0027-8424[print], 0027-8424[linking]
Statut éditorial
Publié
Date de publication
03/1980
Volume
77
Numéro
3
Pages
1437-1441
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
A gene library of Xenopus laevis was constructed from embryonic DNA partially digested with restriction endonucleases Hae III and Alu I and joined to the phage lambda Charon 4 cloning vector with EcoRI linkers. Nucleotide sequences from three of the four related vitellogenin genes have been isolated. Two of the genes (called A1 and A2) were isolated in their entirety together with long stretches of flanking sequences. These two closely related vitellogenin genes have lengths of about 21 and 16 kilobases, but both produce a vitellogenin mRNA of 6.3 kilobases.
Mots-clé
Animals, Bacteriophage lambda/genetics, Base Sequence, Cloning, Molecular/methods, Genes, Lipoproteins/genetics, Molecular Weight, Nucleic Acid Hybridization, Nucleic Acid Precursors/genetics, RNA, Messenger/genetics, Vitellogenins/genetics, Xenopus/genetics
Pubmed
Web of science
Création de la notice
24/01/2008 17:04
Dernière modification de la notice
03/03/2018 19:00
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