The myofibroblast markers α-SM actin and β-actin are differentially expressed in 2 and 3-D culture models of fibrotic and normal skin.

Détails

ID Serval
serval:BIB_86CD5334E40B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
The myofibroblast markers α-SM actin and β-actin are differentially expressed in 2 and 3-D culture models of fibrotic and normal skin.
Périodique
Cytotechnology
Auteur(s)
Vozenin M.C., Lefaix J.L., Ridi R., Biard D.S., Daburon F., Martin M.
ISSN
0920-9069 (Print)
ISSN-L
0920-9069
Statut éditorial
Publié
Date de publication
01/1998
Peer-reviewed
Oui
Volume
26
Numéro
1
Pages
29-38
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
To characterize the differences between fibrotic myofibroblasts and normal fibroblasts, we studied two differentiation markers: α-smooth muscle (SM) actin, a specific marker of myofibroblast differentiation, and β-actin, which is overexpressed in the fibrotic tissue. Experiments were performed on fibroblasts isolated from normal pig skin and on subcutaneous myofibroblasts isolated from pig radiation-induced fibrosis. Three culture models were used: cells in monolayers, equivalent dermis, consisting of fibroblasts embedded into a matrix composed of type I collagen, and in vitro reconstituted skin, in which the matrix and containing life fibroblasts were overlaid with keratinocytes. Samples were studied using immunofluorescence and western-blotting. In monolayers cultures, both fibrosis and normal cells expressed α-SM actin. Furthermore, similar amounts of β-actin protein were found. In these conditions, the resulting alterations in the phenotypes of cells made comparison of cultured fibrotic and normal cells irrelevant. Under the two 3-D culture models, normal fibroblasts no longer expressed α-SM actin. They expressed β-actin at the basal level. Moreover, the fibrotic myofibroblasts in both 3-D models retained their differentiation features, expressing α-SM actin and overexpressing β-actin. We found that this normalization was mainly related to the genomic programmation acquired by the cells in the tissue. Cellular motility and microenvironment were also involved, whereas cellular proliferation was not a major factor. Consequently, both three-dimensional models allowed the study of radiation-induced fibrosis in vitro, provided good extrapolations to in vivo conditions and avoided certain of culture artefacts.

Pubmed
Web of science
Création de la notice
27/04/2018 16:05
Dernière modification de la notice
24/08/2018 6:26
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