Article: article from journal or magazin.
Reconstitution of horse heart cytochrome c: reformation of the peptide bond linking residues 65 and 66.
Biochemical and Biophysical Research Communications
Horse heart cytochrome c can be treated with cyanogen bromide under conditions resulting in division of the molecule into a heme peptide of 65 residues and a peptide of 39 residues, corresponding to segments 1-65 and 66-104 of the parent protein, respectively. Upon mixture of the two peptides in aqueous solution, a complex can be formed that is slowly converted to a product with properties nearly indistinguishable from those of the initial heme protein. Evidence is presented that in this slow phase of the reaction the fragments are rejoined into a continuous chain through restoration of the peptide bond linking residues 65 and 66.
Amino Acid Sequence, Animals, Chromatography, Gel, Cyanogen Bromide, Cytochrome c Group/metabolism, Heme/analysis, Horses, Myocardium/enzymology, Peptide Fragments, Protein Binding
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