Induction of potent antitumor CTL responses by recombinant vaccinia encoding a melan-A peptide analogue.

Details

Serval ID
serval:BIB_85D0492FCC8A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Induction of potent antitumor CTL responses by recombinant vaccinia encoding a melan-A peptide analogue.
Journal
Journal of Immunology
Author(s)
Valmori D., Lévy F., Miconnet I., Zajac P., Spagnoli G.C., Rimoldi D., Liénard D., Cerundolo V., Cerottini J.C., Romero P.
ISSN
0022-1767 (Print)
ISSN-L
0022-1767
Publication state
Published
Issued date
2000
Volume
164
Number
2
Pages
1125-1131
Language
english
Notes
Publication types: Journal Article Publication Status: ppublish
Abstract
There is considerable interest in the development of vaccination strategies that would elicit strong tumor-specific CTL responses in cancer patients. One strategy consists of using recombinant viruses encoding amino acid sequences corresponding to natural CTL-defined peptide from tumor Ags as immunogens. However, studies with synthetic tumor antigenic peptides have demonstrated that introduction of single amino acid substitutions may dramatically increase their immunogenicity. In this study we have used a well-defined human melanoma tumor Ag system to test the possibility of translating the immunological potency of synthetic tumor antigenic peptide analogues into recombinant vaccinia viruses carrying constructs with the appropriate nucleotide substitutions. Our results indicate that the use of a mutated minigene construct directing the expression of a modified melanoma tumor Ag leads to improved Ag recognition and, more importantly, to enhanced immunogenicity. Thus, recombinant vaccinia viruses containing mutated minigene sequences may lead to new strategies for the induction of strong tumor-specific CTL responses in cancer patients.
Keywords
Amino Acid Sequence, Animals, Antigen-Presenting Cells/immunology, Antigens, Neoplasm/administration & dosage, Antigens, Neoplasm/genetics, Cancer Vaccines/administration & dosage, Cancer Vaccines/genetics, Cytotoxicity, Immunologic/genetics, Epitopes, T-Lymphocyte/genetics, Genes, Synthetic/immunology, Genetic Vectors/administration & dosage, Genetic Vectors/chemical synthesis, Humans, Injections, Intraperitoneal, Lymphocyte Activation/genetics, MART-1 Antigen, Melanoma/immunology, Melanoma/therapy, Mice, Mice, Transgenic, Molecular Sequence Data, Mutagenesis, Site-Directed, Neoplasm Proteins/administration & dosage, Neoplasm Proteins/genetics, Peptides/administration & dosage, Peptides/genetics, T-Lymphocytes, Cytotoxic/immunology, Tumor Cells, Cultured, Ubiquitins/genetics, Ubiquitins/immunology, Vaccines, Synthetic/administration & dosage, Vaccines, Synthetic/genetics, Vaccinia virus/genetics, Vaccinia virus/immunology, Viral Vaccines/administration & dosage, Viral Vaccines/chemical synthesis
Pubmed
Web of science
Create date
28/01/2008 11:17
Last modification date
20/08/2019 14:45
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