Microsomal triglyceride transfer protein promotes the secretion of Xenopus laevis vitellogenin A1.

Details

Serval ID
serval:BIB_855D61E25B07
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Microsomal triglyceride transfer protein promotes the secretion of Xenopus laevis vitellogenin A1.
Journal
Journal of Biological Chemistry
Author(s)
Sellers J.A., Hou L., Schoenberg D.R., Batistuzzo de Medeiros S.R., Wahli W., Shelness G.S.
ISSN
0021-9258[print], 0021-9258[linking]
Publication state
Published
Issued date
2005
Peer-reviewed
Oui
Volume
280
Number
14
Pages
13902-13905
Language
english
Notes
Publication types: Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Vitellogenins (Vtg) are ancient lipid transport and storage proteins and members of the large lipid transfer protein (LLTP) gene family, which includes insect apolipophorin II/I, apolipoprotein B (apoB), and the microsomal triglyceride transfer protein (MTP). Lipidation of Vtg occurs at its site of synthesis in vertebrate liver, insect fat body, and nematode intestine; however, the mechanism of Vtg lipid acquisition is unknown. To explore whether Vtg biogenesis requires the apoB cofactor and LLTP family member, MTP, Vtg was expressed in COS cells with and without coexpression of the 97-kDa subunit of human MTP. Expression of Vtg alone gave rise to a approximately 220-kDa apoprotein, which was predominantly confined to an intracellular location. Coexpression of Vtg with human MTP enhanced Vtg secretion by 5-fold, without dramatically affecting its intracellular stability. A comparison of wild type and a triglyceride transfer-defective form of MTP revealed that both were capable of promoting Vtg secretion, whereas only wild type MTP could promote the secretion of apoB41 (amino-terminal 41% of apoB). These studies demonstrate that the biogenesis of Vtg is MTP-dependent and that MTP is the likely ancestral member of the LLTP gene family.
Keywords
Alkaline Phosphatase/genetics, Alkaline Phosphatase/metabolism, Animals, Apolipoproteins B/metabolism, COS Cells, Carrier Proteins/genetics, Carrier Proteins/metabolism, Cercopithecus aethiops, Humans, Vitellogenins/genetics, Vitellogenins/secretion, Xenopus laevis/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:04
Last modification date
20/08/2019 14:44
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