Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion.

Détails

Ressource 1Télécharger: 1078547.pdf (10083.68 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_85015C907BC4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Beneficial Effects of Human Mesenchymal Stromal Cells on Porcine Hepatocyte Viability and Albumin Secretion.
Périodique
Journal of immunology research
Auteur(s)
Montanari E., Pimenta J., Szabó L., Noverraz F., Passemard S., Meier RPH, Meyer J., Sidibe J., Thomas A., Schuurman H.J., Gerber-Lemaire S., Gonelle-Gispert C., Buhler L.H.
ISSN
2314-7156 (Electronic)
ISSN-L
2314-7156
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
2018
Pages
1078547
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Porcine hepatocytes transplanted during acute liver failure might support metabolic functions until the diseased liver recovers its function. Here, we isolated high numbers of viable pig hepatocytes and evaluated hepatocyte functionality after encapsulation. We further investigated whether coculture and coencapsulation of hepatocytes with human multipotent mesenchymal stromal cells (MSC) are beneficial on hepatocyte function. Livers from 10 kg pigs ( <i>n</i> = 9) were harvested, and hepatocytes were isolated from liver suspensions for microencapsulation using alginate and poly(ethylene-glycol)- (PEG-) grafted alginate hydrogels, either alone or in combination with MSC. Viability, albumin secretion, and diazepam catabolism of hepatocytes were measured for one week. 9.2 ± 3.6 × 10 <sup>9</sup> hepatocytes with 95.2 ± 3.1% viability were obtained after isolation. At day 3, free hepatocytes displayed 99% viability, whereas microencapsulation in alginate and PEG-grafted alginate decreased viability to 62% and 48%, respectively. Albumin secretion and diazepam catabolism occurred in free and microencapsulated hepatocytes. Coencapsulation of hepatocytes with MSC significantly improved viability and albumin secretion at days 4 and 8 ( <i>p</i> < 0.05). Coculture with MSC significantly increased and prolonged albumin secretion. In conclusion, we established a protocol for isolation and microencapsulation of high numbers of viable pig hepatocytes and demonstrated that the presence of MSC is beneficial for the viability and function of porcine hepatocytes.
Mots-clé
Albumins/metabolism, Alginates, Animals, Cell Survival, Cells, Cultured, Coculture Techniques, Drug Compounding, Glucuronic Acid, Hepatocytes/physiology, Hepatocytes/transplantation, Hexuronic Acids, Humans, Hydrogels, Liver Failure/therapy, Mesenchymal Stromal Cells/physiology, Swine, Transplantation, Heterologous
Pubmed
Web of science
Open Access
Oui
Création de la notice
23/01/2018 9:13
Dernière modification de la notice
08/05/2019 21:21
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