Influence of neuroblastoma stage on serum-based detection of MYCN amplification.

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Etat: Serval
Version: de l'auteur
ID Serval
serval:BIB_84FA0A941065
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Influence of neuroblastoma stage on serum-based detection of MYCN amplification.
Périodique
Pediatric Blood & Cancer
Auteur(s)
Combaret V., Hogarty M.D., London W.B., McGrady P., Iacono I., Brejon S., Swerts K., Noguera R., Gross N., Rousseau R., Puisieux A.
ISSN
1545-5017[electronic]
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
53
Numéro
3
Pages
329-331
Langue
anglais
Résumé
BACKGROUND: MYCN oncogene amplification has been defined as the most important prognostic factor for neuroblastoma (NB), the most common solid extracranial neoplasm in children. High copy numbers are strongly associated with rapid tumor progression and poor outcome, independently of tumor stage or patient age, and this has become an important factor in treatment stratification. PROCEDURE: By real-time quantitative PCR analysis, we evaluated the clinical relevance of circulating MYCN DNA of 267 patients with locoregional or metastatic NB in children less than 18 months of age. RESULTS: For patients in this age group with INSS stage 4 or 4S NB and stage 3 patients, serum-based determination of MYCN DNA sequences had good sensitivity (85%, 83%, and 75% respectively) and high specificity (100%) when compared to direct tumor gene determination. In contrast, the approach showed low sensitivity patients with stages 1 and 2 disease. CONCLUSION: Our results show that the sensitivity of the serum-based MYCN DNA sequence determination depends on the stage of the disease. However, this simple, reproducible assay may represent a reasonably sensitive and very specific tool to assess tumor MYCN status in cases with stage 3 and metastatic disease for whom a wait and see strategy is often recommended.
Mots-clé
Child, DNA, Neoplasm/blood, Gene Amplification, Genes, myc, Humans, Neoplasm Staging, Neuroblastoma/genetics, Neuroblastoma/pathology, Retrospective Studies
Pubmed
Web of science
Création de la notice
09/02/2010 10:21
Dernière modification de la notice
03/03/2018 18:56
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