Nanopore detection of single molecule RNAP-DNA transcription complex.

Détails

ID Serval
serval:BIB_846A9ACCE57B
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Lettre (letter): communication adressée à l'éditeur.
Collection
Publications
Titre
Nanopore detection of single molecule RNAP-DNA transcription complex.
Périodique
Nano Letters
Auteur(s)
Raillon C., Cousin P., Traversi F., Garcia-Cordero E., Hernandez N., Radenovic A.
ISSN
1530-6992 (Electronic)
ISSN-L
1530-6984
Statut éditorial
Publié
Date de publication
2012
Volume
12
Numéro
3
Pages
1157-1164
Langue
anglais
Résumé
In the past decade, a number of single-molecule methods have been developed with the aim of investigating single protein and nucleic acid interactions. For the first time we use solid-state nanopore sensing to detect a single E. coli RNAP-DNA transcription complex and single E. coli RNAP enzyme. On the basis of their specific conductance translocation signature, we can discriminate and identify between those two types of molecular translocations and translocations of bare DNA. This opens up a new perspectives for investigating transcription processes at the single-molecule level.
Pubmed
Web of science
Création de la notice
05/05/2012 16:55
Dernière modification de la notice
20/08/2019 15:44
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