SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae.

Details

Serval ID
serval:BIB_8461B14D48C3
Type
Article: article from journal or magazin.
Collection
Publications
Title
SMC is recruited to oriC by ParB and promotes chromosome segregation in Streptococcus pneumoniae.
Journal
Molecular Microbiology
Author(s)
Minnen A., Attaiech L., Thon M., Gruber S., Veening J.W.
ISSN
1365-2958 (Electronic)
ISSN-L
0950-382X
Publication state
Published
Issued date
2011
Peer-reviewed
Oui
Volume
81
Number
3
Pages
676-688
Language
english
Abstract
Segregation of replicated chromosomes is an essential process in all organisms. How bacteria, such as the oval-shaped human pathogen Streptococcus pneumoniae, efficiently segregate their chromosomes is poorly understood. Here we show that the pneumococcal homologue of the DNA-binding protein ParB recruits S. pneumoniae condensin (SMC) to centromere-like DNA sequences (parS) that are located near the origin of replication, in a similar fashion as was shown for the rod-shaped model bacterium Bacillus subtilis. In contrast to B. subtilis, smc is not essential in S. pneumoniae, and Δsmc cells do not show an increased sensitivity to gyrase inhibitors or high temperatures. However, deletion of smc and/or parB results in a mild chromosome segregation defect. Our results show that S. pneumoniae contains a functional chromosome segregation machine that promotes efficient chromosome segregation by recruitment of SMC via ParB. Intriguingly, the data indicate that other, as of yet unknown mechanisms, are at play to ensure proper chromosome segregation in this organism.
Keywords
Bacterial Proteins/genetics, Bacterial Proteins/metabolism, Cell Cycle Proteins/genetics, Cell Cycle Proteins/metabolism, Chromosome Segregation, Chromosomes, Bacterial/metabolism, DNA-Binding Proteins/metabolism, Gene Deletion, Origin Recognition Complex, Protein Binding, Streptococcus pneumoniae/enzymology, Streptococcus pneumoniae/genetics
Pubmed
Web of science
Open Access
Yes
Create date
17/08/2016 9:52
Last modification date
20/08/2019 14:44
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