Radiolabeled chimeric anti-CEA monoclonal antibody compared with the original mouse monoclonal antibody for surgically treated colorectal carcinoma.

Détails

ID Serval
serval:BIB_845A9C94400B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Radiolabeled chimeric anti-CEA monoclonal antibody compared with the original mouse monoclonal antibody for surgically treated colorectal carcinoma.
Périodique
Journal of Nuclear Medicine
Auteur(s)
Buchegger F., Mach J.P., Pèlegrin A., Gillet M., Vogel C.A., Buclin T., Ryser J.E., Delaloye B., Delaloye A.B.
ISSN
0161-5505 (Print)
ISSN-L
0161-5505
Statut éditorial
Publié
Date de publication
1995
Volume
36
Numéro
3
Pages
420-429
Langue
anglais
Résumé
Biodistribution and tumor uptake of a chimeric human-mouse monoclonal antibody (MAb) and the original mouse MAb have been comparatively studied.
METHODS: Eighteen patients with suspected colorectal cancer scheduled for surgery underwent immunoscintigraphy with 123I-labeled chimeric anti-CEA MAb. Iodine-125 and 131I trace-labeled chimeric and original mouse MAb were simultaneously injected for biodistribution studies.
RESULTS: Similar serum kinetics and a low immunogenicity were observed for both antibodies. Mean binding capacity to CEA measured in PBS after radiolabeling was identical for both MAbs and it was slightly decreased when measured in serum 1-4 hr after injection. Radiochromatograms of patients sera showed immune complex formation related to the amount of circulating CEA. Postoperative ex vivo radioactivity counting in tissue samples revealed similar antibody distributions with notably similar antibody uptakes in tumors. High tumor uptakes (between 0.02 to 0.06% injected dose per g) were observed in 3 of 13 patients operated for primary or metastatic colorectal cancer.
CONCLUSION: In this dual-label technique, the radioiodinated anti-CEA IgG4 chimeric MAb and the original mouse IgG1 MAb were shown to have very similar behavior in colorectal cancer patients.
Mots-clé
Adult, Aged, Aged, 80 and over, Animals, Antibodies, Monoclonal/diagnostic use, Antibodies, Monoclonal/pharmacokinetics, Carcinoembryonic Antigen/immunology, Colorectal Neoplasms/metabolism, Colorectal Neoplasms/radionuclide imaging, Female, Humans, Male, Mice, Middle Aged, Radioimmunodetection, Recombinant Fusion Proteins/diagnostic use, Recombinant Fusion Proteins/pharmacokinetics
Pubmed
Web of science
Création de la notice
21/01/2008 12:14
Dernière modification de la notice
03/03/2018 18:54
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