Independent evolution of hypervariable regions of HIV-1 gp120: V4 as a swarm of N-Linked glycosylation variants

Détails

ID Serval
serval:BIB_83F70B875C65
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Independent evolution of hypervariable regions of HIV-1 gp120: V4 as a swarm of N-Linked glycosylation variants
Périodique
AIDS Research and Human Retroviruses
Auteur(s)
Castro E., Belair M., Rizzardi G. P., Bart P. A., Pantaleo G., Graziosi C.
ISSN
0889-2229
Statut éditorial
Publié
Date de publication
2008
Peer-reviewed
Oui
Volume
24
Numéro
1
Pages
106-113
Langue
anglais
Résumé
In this study we have characterized intra-patient length polymorphism in V4 by cloning and sequencing a C2-C4 fragment from HIV plasma RNA in patients at different stages of HIV disease. Clonal analysis of clade B, G, and CRF02 isolates during early infection shows extensive intra-patient V4 variability, due to the presence of indel-associated polymorphism. Indels, coupled to amino acid substitution events, affect the number and distribution of potential N-glycosylation sites, resulting in the coexistence, within the same patient, of V4 subsets, each characterized by different sizes, amino acid sequences, and potential N-glycosylation patterns. In contrast, V3 appears to be relatively homogeneous, with similar V3 associated to significantly different V4 within the same clinical specimen. Based on these data, we propose that during early chronic infection V4 is present as a highly divergent quasispecies, enabling the virus to adopt different conformational structures according to immune constrains and other selective pressures
Mots-clé
Acute Disease , Affect , Amino Acid Sequence , Amino Acid Substitution , analysis , Anti-HIV Agents , chemistry , Chronic Disease , drug therapy , Evolution,Molecular , genetics , Glycosylation , HIV Envelope Protein gp120 , HIV Infections , Hiv-1 , Humans , INDEL Mutation , Infection , metabolism , Molecular Sequence Data , Patients , Peptide Fragments , Phylogeny , Plasma , Polymorphism,Genetic , Rna , Switzerland , therapeutic use , virology
Pubmed
Web of science
Création de la notice
29/01/2009 23:13
Dernière modification de la notice
03/03/2018 18:53
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