Transgenic expression of the Ly49A natural killer cell receptor confers class I major histocompatibility complex (MHC)-specific inhibition and prevents bone marrow allograft rejection.

Details

Serval ID
serval:BIB_83F2853A32BD
Type
Article: article from journal or magazin.
Collection
Publications
Title
Transgenic expression of the Ly49A natural killer cell receptor confers class I major histocompatibility complex (MHC)-specific inhibition and prevents bone marrow allograft rejection.
Journal
The Journal of experimental medicine
Author(s)
Held W., Cado D., Raulet D.H.
ISSN
0022-1007
Publication state
Published
Issued date
1996
Peer-reviewed
Oui
Volume
184
Number
5
Pages
2037-2041
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Natural killer (NK) cells and some T cells are endowed with receptors specific for class I major histocompatibility complex (MHC) molecules that can inhibit cellular effector functions. The function of the Ly49 receptor family has been studied in vitro, but no gene transfer experiments have directly established the role of these receptors in NK cell functions. We show here that transgenic expression of the H-2Dd-specific Ly49A receptor in all NK cells and T cells conferred class I-specific inhibition of NK cell-mediated target cell lysis as well as of T cell proliferation. Furthermore, transgene expression prevented NK cell-mediated rejection of allogeneic H-2d bone marrow grafts by irradiated mice. These results demonstrate the function and specificity of Ly49 receptors in vivo, and establish that their subset-specific expression is necessary for the discrimination of MHC-different cells by NK cells in unmanipulated mice.
Keywords
Animals, Antigens, Ly, Bone Marrow Transplantation/immunology, CD4-Positive T-Lymphocytes/immunology, Cytotoxicity, Immunologic, Graft Rejection/immunology, Graft Rejection/prevention &amp, control, H-2 Antigens/immunology, Histocompatibility Antigens Class I/immunology, Killer Cells, Natural/immunology, Lectins, C-Type, Lymphocytes/immunology, Major Histocompatibility Complex/immunology, Membrane Glycoproteins/genetics, Membrane Glycoproteins/immunology, Mice, Mice, Inbred C57BL, Mice, Transgenic, NK Cell Lectin-Like Receptor Subfamily A, Receptors, Immunologic/genetics, Receptors, Immunologic/immunology, Receptors, NK Cell Lectin-Like, Transplantation, Homologous
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2008 15:24
Last modification date
20/08/2019 14:43
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