Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.

Détails

ID Serval
serval:BIB_83938BCEE043
Type
Article: article d'un périodique ou d'un magazine.
Sous-type
Synthèse (review): revue aussi complète que possible des connaissances sur un sujet, rédigée à partir de l'analyse exhaustive des travaux publiés.
Collection
Publications
Titre
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification.
Périodique
Clinical Microbiology and Infection
Auteur(s)
Bizzini A., Greub G.
ISSN
1469-0691[electronic], 1198-743X[linking]
Statut éditorial
Publié
Date de publication
2010
Volume
16
Numéro
11
Pages
1614-1619
Langue
anglais
Résumé
Until recently, matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) techniques for the identification of microorganisms remained confined to research laboratories. In the last 2 years, the availability of relatively simple to use MALDI-TOF MS devices, which can be utilized in clinical microbiology laboratories, has changed the laboratory workflows for the identification of pathogens. Recently, the first prospective studies regarding the performance in routine bacterial identification showed that MALDI-TOF MS is a fast, reliable and cost-effective technique that has the potential to replace and/or complement conventional phenotypic identification for most bacterial strains isolated in clinical microbiology laboratories. For routine bacterial isolates, correct identification by MALDI-TOF MS at the species level was obtained in 84.1-93.6% of instances. In one of these studies, a protein extraction step clearly improved the overall valid identification yield, from 70.3% to 93.2%. This review focuses on the current state of use of MALDI-TOF MS for the identification of routine bacterial isolates and on the main difficulties that may lead to erroneous or doubtful identifications.
Mots-clé
Bacterial identification, clinical microbiology laboratory, matrix-assisted laser desorption ionization time-of-flight mass spectrometry, review, routine samples, resistant staphylococcus-aureus, maldi-tof ms, rapid identification, species identification, routine identification, intact microorganisms, bacteria, discrimination, mycobacteria, streptococci
Pubmed
Web of science
Création de la notice
04/11/2010 11:27
Dernière modification de la notice
03/03/2018 18:53
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