Derivation and Utilization of Functional CD8(+) Dendritic Cell Lines.

Détails

ID Serval
serval:BIB_8387433520A3
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Derivation and Utilization of Functional CD8(+) Dendritic Cell Lines.
Périodique
Methods in Molecular Biology
Auteur(s)
Pigni M., Ashok D., Acha-Orbea H.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2016
Peer-reviewed
Oui
Volume
1423
Pages
39-49
Langue
anglais
Résumé
It is notoriously difficult to obtain large quantities of non-activated dendritic cells ex vivo. For this reason, we produced and characterized a mouse model expressing the large T oncogene under the CD11c promoter (Mushi mice), in which CD8α(+) dendritic cells transform after 4 months. We derived a variety of stable cell lines from these primary lines. These cell lines reproducibly share with freshly isolated dendritic cells most surface markers, mRNA and protein expression, and all tested biological functions. Cell lines can be derived from various strains and knockout mice and can be easily transduced with lentiviruses. In this article, we describe the derivation, culture, and lentiviral transduction of these dendritic cell lines.
Mots-clé
Dendritic cell, Cell line, Tissue culture, Ex vivo derivation
Pubmed
Web of science
Création de la notice
17/05/2016 18:47
Dernière modification de la notice
20/08/2019 15:43
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