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Maintenance of neuronal expression of calbindin by a muscular extract in cultures of chick dorsal root ganglion cells.
Calbindin D-28K is a calcium-binding protein which is expressed by subpopulations of dorsal root ganglion cells cultured from 10-day-old (E10) chick embryos. After 7 or 10 days of culture, more than 20% of the ganglion cells are immunostained by an anticalbindin-antiserum; however, after 14 days of culture, the proportion drops to 10%. This fall can be prevented by addition of muscle extract to cultures at 10 days. Thus the transitory expression of calbindin-immunoreactivity by responsive sensory neurons would be not only induced but also maintained by a differentiation factor of muscular origin.
Animals, Calcium-Binding Protein, Vitamin D-Dependent, Cells, Cultured, Chick Embryo, Ganglia, Spinal, Gene Expression Regulation, Muscles, Time Factors, Tissue Extracts
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