Gene profiling analysis of the early effects of ablative fractional carbon dioxide laser treatment on human skin.

Détails

ID Serval
serval:BIB_837CBC1D9B50
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Gene profiling analysis of the early effects of ablative fractional carbon dioxide laser treatment on human skin.
Périodique
Dermatologic Surgery
Auteur(s)
Kim J.E., Won C.H., Bak H., Kositratna G., Manstein D., Dotto G.P., Chang S.E.
ISSN
1524-4725 (Electronic)
ISSN-L
1076-0512
Statut éditorial
Publié
Date de publication
2013
Peer-reviewed
Oui
Volume
39
Numéro
7
Pages
1033-1043
Langue
anglais
Résumé
BACKGROUND: The use of carbon dioxide (CO2) laser-mediated ablative fractional resurfacing (AFR) is currently under extensive clinical investigation, but the molecular mechanisms underlying this process are unclear.
OBJECTIVES: To determine the early expressed genes that are upregulated in human skin after treatment using a CO2 fractional laser.
METHODS: Whole human skin was irradiated using an AFR CO2 laser, and changes in gene expression after 2 and 24 hours were analyzed using microarray analysis. The results were validated using reverse transcriptase polymerase chain reaction (RT-PCR). Laser scanning confocal microscopy (LSCM) was used to investigate the expression of the validated proteins after AFR CO2 laser treatment of skin that had been biopsied from seven Korean patients.
RESULTS: Gene expression profiling showed that the most significantly upregulated genes in these skin samples were those encoding Wnt5a, cysteine-rich angiogenic inducer 61 (CYR61), and heat shock protein (HSP) 90. These results were confirmed using real-time RT-PCR and LSCM.
CONCLUSIONS: Irradiation using an AFR laser may induce the expression of Wnt5a, CYR61, and HSP90 in human skin during the early remodeling phases, suggesting that the induction of proteins may be the preceding event that is associated with the clinical effects of laser treatment.
Mots-clé
Carbon Dioxide, Collagen/metabolism, Cysteine-Rich Protein 61/metabolism, Dermabrasion, HSP90 Heat-Shock Proteins/metabolism, Humans, Laser Therapy/methods, Matrix Metalloproteinases/metabolism, Microscopy, Confocal, Proto-Oncogene Proteins/metabolism, Skin/radiation effects, Tissue Array Analysis, Up-Regulation/physiology, Wnt Proteins/metabolism, Wound Healing/physiology
Pubmed
Création de la notice
24/09/2014 9:25
Dernière modification de la notice
03/03/2018 18:52
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