Toxic effects of brake wear particles on epithelial lung cells in vitro

Détails

Ressource 1Télécharger: BIB_834D29655A82.P001.pdf (1044.33 [Ko])
Etat: Serval
Version: Final published version
ID Serval
serval:BIB_834D29655A82
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Toxic effects of brake wear particles on epithelial lung cells in vitro
Périodique
Particle and Fibre Toxicology
Auteur(s)
Gasser Michael, Riediker Michael, Mueller Loretta, Perrenoud Alain, Blank Fabian, Gehr Peter, Rothen-Rutishauser Barbara
ISSN
1743-8977 (Electronic)
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
6
Numéro
30
Pages
[13]
Langue
anglais
Notes
SAPHIRID:81301
Résumé
Background: Fine particulate matter originating from traffic correlates with increased morbidity and mortality. An important source of traffic particles is brake wear of cars which contributes up to 20% of the total traffic emissions. The aim of this study was to evaluate potential toxicological effects of human epithelial lung cells exposed to freshly generated brake wear particles. Results: An exposure box was mounted around a car's braking system. Lung cells cultured at the air-liquid interface were then exposed to particles emitted from two typical braking behaviours ("full stop" and "normal deceleration"). The particle size distribution as well as the brake emission components like metals and carbons was measured on-line, and the particles deposited on grids for transmission electron microscopy were counted. The tight junction arrangement was observed by laser scanning microscopy. Cellular responses were assessed by measurement of lactate dehydrogenase (cytotoxicity), by investigating the production of reactive oxidative species and the release of the pro-inflammatory mediator interleukin-8. The tight junction protein occludin density decreased significantly (p < 0.05) with increasing concentrations of metals on the particles (iron, copper and manganese, which were all strongly correlated with each other). Occludin was also negatively correlated with the intensity of reactive oxidative species. The concentrations of interleukin-8 were significantly correlated with increasing organic carbon concentrations. No correlation was observed between occludin and interleukin-8, nor between reactive oxidative species and interleukin-8. Conclusion: These findings suggest that the metals on brake wear particles damage tight junctions with a mechanism involving oxidative stress. Brake wear particles also increase pro-inflammatory responses. However, this might be due to another mechanism than via oxidative stress. [Authors]
Mots-clé
Vehicle Emissions , Particulate Matter , Particle Size , Epithelial Cells , Toxicity Tests
Pubmed
Web of science
Création de la notice
19/01/2010 18:30
Dernière modification de la notice
03/03/2018 18:52
Données d'usage