STEAP, a prostate tumor antigen, is a target of human CD8+ T cells

Détails

ID Serval
serval:BIB_82BD1450E2DE
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
STEAP, a prostate tumor antigen, is a target of human CD8+ T cells
Périodique
Cancer Immunology, Immunotherapy
Auteur(s)
Alves  P. M., Faure  O., Graff-Dubois  S., Cornet  S., Bolonakis  I., Gross  D. A., Miconnet  I., Chouaib  S., Fizazi  K., Soria  J. C., Lemonnier  F. A., Kosmatopoulos  K.
ISSN
0340-7004 (Print)
Statut éditorial
Publié
Date de publication
12/2006
Volume
55
Numéro
12
Pages
1515-23
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Dec
Résumé
STEAP is a recently identified protein shown to be particularly overexpressed in prostate cancer and also present in numerous human cancer cell lines from prostate, pancreas, colon, breast, testicular, cervical, bladder and ovarian carcinoma, acute lymphocytic leukemia and Ewing sarcoma. This expression profile renders STEAP an appealing candidate for broad cancer immunotherapy. In order to investigate if STEAP is a tumor antigen that can be targeted by specific CD8(+) T cells, we identified two high affinity HLA-A*0201 restricted peptides (STEAP(86-94) and STEAP(262-270)). These peptides were immunogenic in vivo in HLA-A*0201 transgenic HHD mice. Peptide specific murine CD8 T cells recognized COS-7 cells co-transfected with HHD (HLA-A*0201) and STEAP cDNA constructs and also HLA-A*0201(+) STEAP(+) human tumor cells. Furthermore, STEAP(86-94) and STEAP(262-270) stimulated specific CD8(+) T cells from HLA-A*0201(+) healthy donors, and these peptide specific CD8(+) T cells recognized STEAP positive human tumor cells in an HLA-A*0201-restricted manner. Importantly, STEAP(86-94)-specific T cells were detected and reactive in the peripheral blood mononuclear cells in NSCLC and prostate cancer patients ex vivo. These results show that STEAP can be a target of anti-tumor CD8(+) T cells and that STEAP peptides can be used for a broad-spectrum-tumor immunotherapy.
Mots-clé
Amino Acid Sequence Animals Antigens, Neoplasm/*immunology Carcinoma, Non-Small-Cell Lung/immunology Cell Line, Tumor HLA-A Antigens/genetics Humans Lung Neoplasms/immunology Male Mice Mice, Transgenic Molecular Sequence Data Peptide Fragments/*immunology T-Lymphocytes, Cytotoxic/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:08
Dernière modification de la notice
03/03/2018 18:51
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