Ardiscretin a novel arthropod-selective toxin from Tityus discrepans scorpion venom.

Détails

ID Serval
serval:BIB_825ECD0E1FAB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Ardiscretin a novel arthropod-selective toxin from Tityus discrepans scorpion venom.
Périodique
Toxicon
Auteur(s)
D'Suze G., Sevcik C., Corona M., Zamudio F.Z., Batista C.V., Coronas F.I., Possani L.D.
ISSN
0041-0101
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
43
Numéro
3
Pages
263-272
Langue
anglais
Résumé
A new arthropod selective toxin was purified from the venom of the Venezuelan scorpion Tityus discrepans, and its amino acid sequence, cDNA clone and biological activity are reported here. The amino acid sequence of this peptide, named ardiscretin (from arthropod toxin of T. discrepans) was completed by Edman degradation and mass spectrometry. It is a single polypeptide composed by 61 amino acids with an amidated cysteine residue at the C-terminal end, closely packed by four disulfide bridges. The atomic mass unit (a.m.u.) experimentally determined was 7103.8 a.m.u. This peptide was shown to be specific for invertebrates (crickets, triatomides, crabs and squids), but non-toxic to mice, at the dose assayed. Ardiscretin inhibits the Na(+)-currents of squid giant axons in an apparent irreversible manner, whose inhibitory effect is reached at 30 microM toxin concentration. Sequence comparison showed that it is phylogenetically closely related to insect-specific scorpion toxins. Ardiscretin produced a small depolarization and induced repetitive firing in squid axons resembling those of DDT [1,1'(p-chlorobenzyl)2-tricloretane] in its ability to slow down action potential, to induce repetitive firing, and in that the concentration required for any effect in squid axon is rather high.
Mots-clé
Amino Acid Sequence, Animals, Arthropods/drug effects, Base Sequence, DNA Primers, Male, Mice, Molecular Sequence Data, Neurotoxins/chemistry, Neurotoxins/genetics, Phylogeny, Polymerase Chain Reaction, Scorpion Venoms/chemistry, Scorpion Venoms/genetics, Sequence Alignment, Sodium Channels/drug effects
Pubmed
Web of science
Création de la notice
10/03/2008 12:44
Dernière modification de la notice
03/03/2018 18:50
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