Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.

Détails

ID Serval
serval:BIB_8258ED09645A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Noninvasive characterization of the fission yeast cell cycle by monitoring dry mass with digital holographic microscopy.
Périodique
Journal of biomedical optics
Auteur(s)
Rappaz B., Cano E., Colomb T., Kühn J., Depeursinge C., Simanis V., Magistretti P.J., Marquet P.
ISSN
1083-3668
Statut éditorial
Publié
Date de publication
2009
Peer-reviewed
Oui
Volume
14
Numéro
3
Pages
034049
Langue
anglais
Notes
Publication types: Journal Article - Publication Status: ppublish
Résumé
Digital holography microscopy (DHM) is an optical technique which provides phase images yielding quantitative information about cell structure and cellular dynamics. Furthermore, the quantitative phase images allow the derivation of other parameters, including dry mass production, density, and spatial distribution. We have applied DHM to study the dry mass production rate and the dry mass surface density in wild-type and mutant fission yeast cells. Our study demonstrates the applicability of DHM as a tool for label-free quantitative analysis of the cell cycle and opens the possibility for its use in high-throughput screening.
Pubmed
Web of science
Création de la notice
09/07/2009 14:49
Dernière modification de la notice
03/03/2018 18:50
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