Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization

Détails

ID Serval
serval:BIB_825778D13B21
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Reconstitution of telomerase activity combined with HPV-E7 expression allow human preadipocytes to preserve their differentiation capacity after immortalization
Périodique
Cell Death and Differentiation
Auteur(s)
Darimont  C., Zbinden  I., Avanti  O., Leone-Vautravers  P., Giusti  V., Burckhardt  P., Pfeifer  A. M., Mace  K.
ISSN
1350-9047 (Print)
Statut éditorial
Publié
Date de publication
09/2003
Volume
10
Numéro
9
Pages
1025-31
Notes
Journal Article --- Old month value: Sep
Résumé
Overexpression of SV40 T-antigen (SV40 T-Ag) has been widely used to overcome replicative senescence of human primary cells and to promote cell immortalization. However, in the case of certain cell types, such as preadipocytes, the differentiation process of immortalized cells is blocked by SV40 T-Ag expression. In this study, human telomerase reverse transcriptase (hTERT) and papillomavirus E7 oncoprotein (HPV-E7) genes were coexpressed in human preadipocytes to test whether this combination could maintain cell differentiation capacity after immortalization. We demonstrated that the HPV-E7/hTERT expressing preadipocytes displayed an indefinite life span. Interestingly, immortalized cells were diploid and presented no chromosomic alterations. These immortalized cells were able to accumulate and hydrolyze intracellular triglycerides and to express adipocyte markers. These data demonstrate, for the first time, that coexpression of hTERT and HPV-E7 in human preadipocytes allows cells not only to display an indefinite life span but also to retain their capacity to differentiate.
Mots-clé
Adipocytes/*cytology/metabolism Adult Cell Differentiation Cell Line, Transformed *Cell Transformation, Viral Cells, Cultured DNA-Binding Proteins Female Gene Expression Humans Karyotyping Oncogene Proteins, Viral/genetics/*metabolism Stem Cells/*cytology/enzymology/virology Telomerase/*metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 17:23
Dernière modification de la notice
08/05/2019 21:12
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