Barrier to autointegration factor interacts with the cone-rod homeobox and represses its transactivation function.

Détails

ID Serval
serval:BIB_8212ECABF617
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Barrier to autointegration factor interacts with the cone-rod homeobox and represses its transactivation function.
Périodique
Journal of Biological Chemistry
Auteur(s)
Wang X., Xu S., Rivolta C., Li L.Y., Peng G.H., Swain P.K., Sung C.H., Swaroop A., Berson E.L., Dryja T.P., Chen S.
ISSN
0021-9258
Statut éditorial
Publié
Date de publication
11/2002
Peer-reviewed
Oui
Volume
277
Numéro
45
Pages
43288-43300
Langue
anglais
Résumé
Crx (cone-rod homeobox) is a homeodomain transcription factor implicated in regulating the expression of photoreceptor and pineal genes. To identify proteins that interact with Crx in the retina, we carried out a yeast two-hybrid screen of a retinal cDNA library. One of the identified clones encodes Baf (barrier to autointegration factor), which was previously shown to have a role in mitosis and retroviral integration. Additional biochemical assays provided supporting evidence for a Baf-Crx interaction. The Baf protein is detectable in all nuclear layers of the mouse retina, including the photoreceptors and the bipolar cells where Crx is expressed. Transient transfection assays with a rhodopsin-luciferase reporter in HEK293 cells demonstrate that overexpression of Baf represses Crx-mediated transactivation, suggesting that Baf acts as a negative regulator of Crx. Consistent with this role for Baf, an E80A mutation of CRX associated with cone-rod dystrophy has a higher than normal transactivation potency but a reduced interaction with Baf. Although our studies did not identify a causative Baf mutation in retinopathies, we suggest that Baf may contribute to the phenotype of a photoreceptor degenerative disease by modifying the activity of Crx. In view of the ubiquitous expression of Baf, we hypothesize that it may play a role in regulating tissue- or cell type-specific gene expression by interacting with homeodomain transcription factors.
Mots-clé
Amino Acid Sequence, Animals, Base Sequence, Cattle, Cell Line, Cloning, Molecular, Computational Biology, DNA Primers, DNA-Binding Proteins/genetics, DNA-Binding Proteins/metabolism, Gene Library, Homeodomain Proteins/metabolism, Humans, Mice, Molecular Sequence Data, Nuclear Proteins, Polymerase Chain Reaction, Rats, Retina/metabolism, Saccharomyces cerevisiae/genetics, Sequence Alignment, Sequence Homology, Amino Acid, Species Specificity, Trans-Activators/metabolism, Transcriptional Activation, Transfection
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 15:14
Dernière modification de la notice
08/05/2019 21:12
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