Article: article from journal or magazin.
hShroom1 links a membrane bound protein to the actin cytoskeleton.
Cellular and molecular life sciences : CMLS
hShroom1 (hShrm1) is a member of the Apx/Shroom (Shrm) protein family and was identified from a yeast two-hybrid screen as a protein that interacts with the cytoplasmic domain of melanoma cell adhesion molecule (MCAM). The characteristic signature of the Shrm family is the presence of a unique domain, ASD2 (Apx/Shroom domain 2). mRNA analysis suggests that hShrm1 is expressed in brain, heart, skeletal muscle, colon, small intestine, kidney, placenta and lung tissue, as well a variety of melanoma and other cell lines. Co-immunoprecipitation and bioluminescence resonance energy transfer (BRET) experiments indicate that hShrm1 and MCAM interact in vivo and by immunofluorescence microscopy some co-localization of these proteins is observed. hShrm1 partly co-localises with beta-actin and is found in the Triton X-100 insoluble fraction of melanoma cell extracts. We propose that hShrm1 is involved in linking MCAM to the cytoskeleton.
Actins/metabolism, Alternative Splicing, Amino Acid Sequence, Animals, Antigens, CD146/genetics, Antigens, CD146/metabolism, Cell Line, Cytoskeleton/metabolism, Humans, Melanoma/genetics, Melanoma/metabolism, Membrane Proteins/genetics, Membrane Proteins/metabolism, Microfilament Proteins/genetics, Microfilament Proteins/metabolism, Molecular Sequence Data, Protein Isoforms/genetics, Protein Isoforms/metabolism, Recombinant Fusion Proteins/genetics, Recombinant Fusion Proteins/metabolism, Two-Hybrid System Techniques
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