Cellular location of the cleavage event of the polymeric immunoglobulin receptor and fate of its anchoring domain in the rat hepatocyte

Détails

ID Serval
serval:BIB_81EAE7E3A419
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Cellular location of the cleavage event of the polymeric immunoglobulin receptor and fate of its anchoring domain in the rat hepatocyte
Périodique
Biochemical Journal
Auteur(s)
Solari  R., Schaerer  E., Tallichet  C., Braiterman  L. T., Hubbard  A. L., Kraehenbuhl  J. P.
ISSN
0264-6021 (Print)
Statut éditorial
Publié
Date de publication
02/1989
Volume
257
Numéro
3
Pages
759-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Feb 1
Résumé
Transcytosis of polymeric immunoglobulin (pIg) across glandular and mucosal epithelia is mediated by a member of the immunoglobulin supergene family, the pIg receptor. During transcellular routing, the receptor is cleaved and its ectoplasmic domain, known as secretory component (SC), is released into secretions bound to pIg. Using receptor-domain-specific antibodies, we have combined cell fractionation and immunoblotting of rat liver to examine the cellular routing of the receptor, the cellular location of the cleavage event and the fate of the anchor domain. Cleavage is a late event in receptor processing. It appears to occur at the canalicular plasma membrane, since intact receptor is present in this membrane domain and no SC is detected in whole liver homogenate or in cell fractions. The membrane anchor remaining after cleavage can be recovered in bile, as well as in a low-density fraction obtained after equilibrium centrifugation of liver (microsomal fractions) on sucrose density gradients. These data suggest that the membrane-anchor domain may be internalized as well as secreted together with SC into bile.
Mots-clé
Animals Bile/analysis Cell Membrane/metabolism Female Immunoglobulin A/*metabolism Liver/cytology/*immunology Male Polymers Rats Rats, Inbred Strains Receptors, Immunologic/*analysis/metabolism Secretory Component/analysis
Pubmed
Web of science
Création de la notice
25/01/2008 16:05
Dernière modification de la notice
03/03/2018 18:49
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