Characterization of Human Late Outgrowth Endothelial Progenitor-Derived Cells under Various Flow Conditions.

Détails

ID Serval
serval:BIB_81B8D95477AA
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of Human Late Outgrowth Endothelial Progenitor-Derived Cells under Various Flow Conditions.
Périodique
Journal of Vascular Research
Auteur(s)
Mazzolai L., Bouzourene K., Hayoz D., Dignat-George F., Liu J.W., Bounameaux H., Dunoyer-Geindre S., Kruithof E.K.
ISSN
1423-0135 (Electronic)
ISSN-L
1018-1172
Statut éditorial
Publié
Date de publication
2011
Volume
48
Numéro
5
Pages
443-451
Langue
anglais
Résumé
Background: Endothelial progenitor-derived cells (EPC) are a cell therapy tool in peripheral arterial disease and for re-endothelialization of bypasses and stents. Objective: To assess EPC behavior under flow conditions normally found in vivo. Results: EPC were isolated from human cord blood, cultured on compliant tubes and exposed in an in vitro flow system mimicking hemodynamic environments normally found in medium and large arteries. EPC exposed for 24 h to unidirectional (0.3 ± 0.1 or 6 ± 3 dynes/cm(2)) shear stress oriented along flow direction, while those exposed to bidirectional shear stress (0.3 ± 3 dynes/cm(2)) or static conditions had random orientation. Under bidirectional flow, tissue factor (TF) activity and mRNA expression were significantly increased (2.5- and 7.0-fold) compared to static conditions. Under low shear unidirectional flow TF mRNA increased 4.9 ± 0.5-fold. Similar flow-induced increases were observed for TF in mature umbilical vein-derived endothelial cells. Expression of tissue-type plasminogen activator (t-PA), urokinase (u-PA) and monocyte chemotactic protein 1 (MCP1) were reduced by 40-60% in late outgrowth endothelial progenitor-derived cells (LO-EPC) exposed to any flow environment, while MCP1, but not t-PA or u-PA, was decreased in HUVEC. Conclusions: Flow, in particular bidirectional, modifies the hemostatic balance in LO-EPC with increased TF and decreased plasminogen activator expression.
Pubmed
Web of science
Open Access
Oui
Création de la notice
03/09/2011 21:08
Dernière modification de la notice
08/05/2019 21:10
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