Characterization of three novel monoclonal antibodies against hepatitis C virus core protein

Détails

ID Serval
serval:BIB_81B01E2C3396
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Characterization of three novel monoclonal antibodies against hepatitis C virus core protein
Périodique
Journal of Medical Virology
Auteur(s)
Moradpour  D., Wakita  T., Tokushige  K., Carlson  R. I., Krawczynski  K., Wands  J. R.
ISSN
0146-6615 (Print)
Statut éditorial
Publié
Date de publication
03/1996
Volume
48
Numéro
3
Pages
234-41
Notes
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Mar
Résumé
Three novel monoclonal antibodies (MAbs) were established against a recombinant hepatitis C virus (HCV) core protein derived from cloned genotype 1b HCV cDNA. MAbs C7-50 and C8-59 recognize a conserved linear epitope represented by amino acid residues 21 to 40 of the nucleocapsid protein. MAb C8-48 is directed against a strain-specific conformational epitope located within the first 82 amino acids. A sensitive two-site MAb-based immunoradiometric assay was established using antibodies directed against distinct epitopes on the nucleocapsid protein. Processed 21 kDa core protein was detected by immunoblotting in human hepatocellular carcinoma cell lines and primary adult rat hepatocytes transfected with a cytomegalovirus promoter-driven expression construct. Immunofluorescence microscopy studies revealed a granular and vesicular cytoplasmic staining pattern. MAb C7-50 was used successfully to detect HCV core antigen in chronically infected chimpanzee liver tissue. These MAbs represent important reagents for the study of HCV biology and for the development of immunodiagnostic assays.
Mots-clé
Animals Antibodies, Monoclonal/*immunology Binding, Competitive Cell Line Epitope Mapping Fluorescent Antibody Technique, Indirect Hepacivirus/*immunology/isolation & purification Hepatitis C/immunology/virology Hepatitis C Antibodies/*immunology Humans Liver/immunology/virology Mice Mice, Inbred BALB C Microscopy, Fluorescence Pan troglodytes Rats Tumor Cells, Cultured Viral Core Proteins/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 17:05
Dernière modification de la notice
20/08/2019 15:41
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