Detection of JC virus-specific cytotoxic T lymphocytes in healthy individuals.

Détails

ID Serval
serval:BIB_818367D60578
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Detection of JC virus-specific cytotoxic T lymphocytes in healthy individuals.
Périodique
Journal of Virology
Auteur(s)
Du Pasquier R.A., Schmitz J.E., Jean-Jacques J., Zheng Y., Gordon J., Khalili K., Letvin N.L., Koralnik I.J.
ISSN
0022-538X[print], 0022-538X[linking]
Statut éditorial
Publié
Date de publication
2004
Peer-reviewed
Oui
Volume
78
Numéro
18
Pages
10206-10210
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
The polyomavirus JC (JCV) infects 85% of healthy individuals, and its reactivation in a limited number of immunosuppressed people causes progressive multifocal leukoencephalopathy (PML), a severe demyelinating disease of the central nervous system. We hypothesized that JCV-specific cytotoxic T lymphocytes (CTLs) might control JCV replication in healthy individuals, blocking the evolution of PML. Using 51Cr release and tetramer staining assays, we show that 8 of 11 HLA-A*0201+ healthy subjects (73%) harbor detectable JCV-specific CD8+ CTLs that recognize one or two epitopes of JCV VP1 protein, the HLA-A*0201-restricted VP1p36 and VPp1100 epitopes. We determined that the frequency of JCV VP1 epitope-specific CTLs varied from less than 1/100,000 to 1/2,494 peripheral blood mononuclear cells. More individuals had JCV VP1-specific than cytomegalovirus-specific CTLs (8 of 11 subjects [73%] versus 2 of 10 subjects [20%], respectively). These results show that a CD8+-T-cell response against JCV is commonly found in immunocompetent people and suggest that these cells might protect against the development of PML.
Mots-clé
Adult, Cytomegalovirus/immunology, DNA-Binding Proteins/immunology, HLA-A Antigens/metabolism, Humans, Immunocompetence, Immunodominant Epitopes, JC Virus/immunology, JC Virus/pathogenicity, Leukoencephalopathy, Progressive Multifocal/immunology, Leukoencephalopathy, Progressive Multifocal/prevention &amp, control, Plant Proteins, T-Lymphocytes, Cytotoxic/immunology, Trans-Activators, Transcription Factors/immunology, Virus Replication/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
25/01/2008 15:56
Dernière modification de la notice
08/05/2019 21:10
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